Tehran University of Medical Sciences Frontiers in Dentistry 2676-296X 8 1 2011 03 15 1 6 Sh. Abdollahzadeh Assistant Professor, Department of Oral Medicine, School of Dentistry, Hamadan University of Medical RY. Mashouf Professor, Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, H H. Mortazavi Assistant Professor, Department of Oral Medicine, School of Dentistry, Hamadan University of Medical MH. Moghaddam Associate Professor, Traditional Medicine and Materia Medica Research Center, Shahid Beheshti Univer N. Roozbahani Dentist, Private Practice M. Vahedi Assistant Professor, Department of Oral Medicine, School of Dentistry, Hamadan University of Medical 2015 10 03 Objective: Punica granatum has been used for many years in folk medicine due to several purposes. The aim of the present study was to evaluate the effect of methanolic extract of Punica granatum peel (MEPGP) against Streptococcus mutans, Staphylococcus aureus, Streptococcus salivarius, Streptococcus sanguinis, Staphylococcus epidermidis, Actynomyces viscosus, Lactobacillus acidophilus and Candida albicans. Materials and Methods: In this in vitro study, the mentioned oral organisms were cultured in blood agar and mueller-hinton media and then paper disks containing MEPGP at concentrations of 4 mg/ml, 8 mg/ml and 12 mg/ml were inserted on medias. The antimicrobial activity was evaluated by agar disk diffusion method. The effects of three different concentrations of MEPGP against microorganisms were compared using one-way ANOVA and Tukey tests. Results: All concentrations of MEPGP had antibacterial activity against S. aureus and S. epidermidis. Only at concentration of 8 mg/ml and 12 mg/ml MEPGP was effective against L. acidophilus, S. mutans and S. salivarius. Furthermore; no concentrations of MEPGP inhibited A. viscosus and C. albicans. Conclusion: This study suggests that MEPGP might be used as an antibacterial agent in controlling oral infections. https://fid.tums.ac.ir/index.php/fid/article/view/234 https://fid.tums.ac.ir/index.php/fid/article/download/234/234