Tehran University of Medical Sciences Frontiers in Dentistry 2676-296X 16 5 2019 05 29 402 406 EN Sara Pourshahidi Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran Elaheh Ghasemzadeh Hoseini Department of Oral and Maxillofacial Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran Hooman Ebrahimi Oral Medicine Department, Dental Branch, Islamic Azad University, Tehran, Iran Mojgan Alaeddini Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran Shahroo Etemad-Moghadam Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran Shiva Shirazian Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran 2018 04 28 2019 01 09 Induction of premalignant lesions in animal models is of high value for research purposes. This study aimed to induce dysplasia in hamster mucosal pouch for investigation of dysplastic lesions using dimethylbenz(a)anthracene. The buccal pouch of 10 hamsters was painted with dimethylbenz(a)anthracene for 10 weeks every other day. At 5 and 10 weeks, they underwent histopathological analysis. Clinically, there was no change until week 7; after which mucosal thickening occurred. Hamsters scarified at 5 weeks and 10 weeks demonstrated mild and moderate dysplasia, respectively. dimethylbenz(a)anthracene is a useful tool for inducing dysplastic lesions in the buccal pouch mucosa of hamsters. https://fid.tums.ac.ir/index.php/fid/article/view/2534 https://fid.tums.ac.ir/index.php/fid/article/download/2534/1780