Tehran University of Medical Sciences Frontiers in Dentistry 2676-296X 21 0 2024 08 13 1 8 Suji Daviasigamani Department of Prosthodontics. SRM Dental College, Ramapuram, SRM University, Chennai, India Ahila Singaravel Chidambaranathan Department of Prosthodontics. SRM Dental College, Ramapuram, SRM University, Chennai, India Muthukumar Balasubramanium Department of Prosthodontics. SRM Dental College, Ramapuram, SRM University, Chennai, India 2020 06 30 2021 05 01 Objectives: Compare the tensile strength and rupture elongation of room temperature vulcanizing silicone (RTV), heat temperature vulcanizing silicone (HTV) and 3% SiO2 reinforced RTV and HTV following disinfection with various agents. Materials and Methods: According to ASTM D412, 384 samples were fabricated using HTV, RTV, RTV and HTV reinforced with 3% SiO2 nanoparticles. The control group received no disinfection treatment, while the other samples were disinfected for 10 minutes using neutral soap, 4% chlorhexidine, and ozone water, three times a day for 60 days. Additionally, accelerated aging was carried out for 252,504,1008 hours. Tensile strength and rupture elongation were assessed using a universal testing machine at 500 mm/min speed, and the mean values were analyzed using two-way ANOVA and Tukey HSD test (P<0.05). Results: The mean value of tensile strength of RTV (2.96 ± 0.41), 3%SiO2 RTV (3.26 ±0.33), HTV (3.30 ±0.36),3% SiO2 HTV (4.07 ±0.85) MPa which was statistically significant for control, neutral soap and 4% chlorhexidine at 252,504 ,1008 hours of aging. (P <0.05). The percentage of elongation of RTV (545 ±29.2),3%SiO2 RTV (617 ±30.5), HTV (735 ±48.7),3% SiO2 HTV (801 ±55.7) which was statically significant for control, neutral soap, 4% chlorhexidine and Ozone water for 252, 504 ,1008 hours of aging. (P <0.05). Conclusion: The HTV silicone showed more tensile strength and rupture elongation compared to HTV, RTV and RTV silicones reinforced with 3% SiO2 nanoparticles. Ozone water disinfection had least effect on tensile strength and rupture elongation of maxillo-facial silicone compared to other disinfectant. https://fid.tums.ac.ir/index.php/fid/article/view/3646 https://fid.tums.ac.ir/index.php/fid/article/download/3646/2061