Tehran University of Medical Sciences Frontiers in Dentistry 2676-296X 19 0 2022 09 13 3891 3891 Amirarsalan Hooshyarfard Dental Materials Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran AND Department of Periodontics, Faculty of Dentistry, Shahed University, Tehran, Iran Banafsheh Poormoradi Laser Research Center, Department of Periodontics, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran. Fatemeh Olad Private Practice, Ilam, Iran Armaghan Shahbazi Department of Prosthodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Zahra Cheraghi Modeling of Noncommunicable Diseases Research Center, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran. 2020 12 17 2021 11 08 Objectives: Considering the side effects of chlorhexidine (CHX), which is currently the gold-standard antimicrobial mouthwash, this study aimed to compare the effects of Green Kemphor and CHX mouthwashes on tooth staining and gingivitis. Materials and Methods: This randomized controlled crossover clinical trial evaluated 38 patients requiring CHX mouthwash following oral surgery and periodontal therapy. The patients were randomly assigned to CHX and Kemphor groups (n=19). In CHX group, patients used CHX mouthwash in the first 2 weeks, and after a 4-day washout period, they used Kemphor mouthwash for 2 weeks. This order was reverse in the Kemphor group. Gingivitis was evaluated using the Silness and Loe gingival index (GI), and tooth staining was evaluated by the Lobene index at 0 (baseline), 2 and 4 weeks. Data were analyzed by paired t-test. Results: CHX mouthwash significantly decreased the GI and increased tooth staining (gingival stains, body stains, and stain extent) at 2 weeks (P<0.05). Kemphor mouthwash significantly decreased the GI and increased tooth staining after 2 weeks (P<0.05). The GI in Kemphor group was significantly lower than that in CHX group at 4 weeks (P<0.05). Also, the tooth staining parameters in the Kemphor group were significantly lower than the corresponding values in the CHX group at 2 and 4 weeks (P<0.05). Conclusion: Kemphor had higher efficacy for reduction of GI and caused less tooth staining than CHX; thus, it may be recommended for use as an alternative to CHX. https://fid.tums.ac.ir/index.php/fid/article/view/3891