Tehran University of Medical Sciences Frontiers in Dentistry 2676-296X 22 0 2025 02 18 4160 4160 Lakshmi Kalyani Kondepudi Department of Periodontics, Vishnu Dental College Bhimavaram, India Suneetha Koneru Department of Periodontics, Drs Sudha and Nageswararao Siddhartha Institute of Dental Sciences, Chinoutpally, Gannavaram, India C.D. Dwarakanath Department of Periodontics, Vishnu Dental College Bhimavaram, India 2021 08 20 2023 06 05 Objectives: Amniotic membrane (AM) is an allograft obtained from humans that contains a variety of growth factors, and has outstanding healing qualities. This study aimed to assess the efficacy of amniotic allograft with coronally advanced flap (CAF) for root coverage. Materials and Methods: This randomized controlled clinical trial comprised 24 individuals with Miller's Class I and II root resorption defects. Each patient received treatment using the CAF approach, with the test group receiving a combined therapy using an AM. Measurements were made at baseline and 6 months after surgery for gingival biotype (GB), dentin hypersensitivity (DH), recession depth (RD), recession width (RW), gingival recession total surface area (GRTSA), keratinized tissue width (KTW) and probing depth (PD). Results: There was a statistically significant reduction in RD (from 2.83 mm to 0.92 mm), a significant increase in KTW (from 3.17 mm to 4.25 mm), improvement in GB, and a reduction in DH in the test group compared to the control group (P<0.05). Conclusion: Miller’s Class I and II root recession defects can be effectively treated with a CAF. Application of AM under the CAF improved root coverage and GB, and further increased the KTW after 6 months, compared to CAF alone. https://fid.tums.ac.ir/index.php/fid/article/view/4160