Tehran University of Medical Sciences Frontiers in Dentistry 2676-296X 20 0 2023 10 22 1 7 Reza Eftekhar Ashtiani Department of Prosthodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran Elaheh Beyabanaki Department of Prosthodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mahshid Razmgah Private Practice, Tehran, Iran Alma Salazar Comprehensive Dentistry Department, College of Dentistry, Texas A&M University, Dallas, TX Marta Revilla-León Comprehensive Dentistry Department, College of Dentistry, Texas A&M University, Dallas, TX Amirali Zandinejad Comprehensive Dentistry Department, College of Dentistry, Texas A&M University, Dallas, TX 2021 09 20 2022 05 30 Objectives: This in vitro study aimed to compare the effect of external staining agents (black tea and grape juice) on the color change of three hybrid ceramics including a new material in comparison to zirconia-reinforced lithium silicate. Materials and Methods: Ten samples of two hybrid dental ceramics including Vita Enamic (Vita Zahnfabrik) and Crystal Ultra (Digital Dental) in addition to a resin nanoceramic, Lava Ultimate (3M ESPE); and a zirconia-reinforced lithium silicate glass ceramic, Vita Suprinity (Vita Zahnfabrik) were prepared and immersed in black tea or grape juice for 30 days. A spectrophotometer was used to evaluate color change. All samples were carefully chosen in A3 shades or their equivalent. Two-way ANOVA and Bonferroni tests were used to analyze the color differences (ΔE00) among the materials and the staining solutions at a significant level of 0.05. Results: Significant color changes were observed in Vita Enamic and Lava Ultimate groups after immersion in both solutions (P<0.001). The least color changes (ΔE00) belonged to the Vita Suprinity (1.34) and Crystal Ultra (1.92) groups, respectively. Conclusion: The color changes of Vita Enamic and Lava Ultimate materials were beyond clinical acceptability. However, the mean color changes of Vita Suprinity and Crystal Ultra groups were clinically acceptable.  https://fid.tums.ac.ir/index.php/fid/article/view/4201