Tehran University of Medical Sciences Frontiers in Dentistry 2676-296X 22 0 2025 02 18 4277 4277 Abinaya Kutralanathan Department of Prosthodontics and Crown and Bridge, SRM Dental College, Bharathi Salai, Ramapuram, Chennai, Tamil Nadu, India Ahila Singaravel Chidambaranathan Department of Prosthodontics and Crown and Bridge, SRM Dental College, Bharathi Salai, Ramapuram, Chennai, Tamil Nadu, Indi Muthukumar Balasubramaniam Department of Prosthodontics and Crown and Bridge, SRM Dental College, Bharathi Salai, Ramapuram, Chennai, Tamil Nadu, India 2021 11 18 2022 12 21 Objectives: This study evaluated the effect of ozone water disinfection on dimensional stability and accuracy of silicone impression materials. Materials and Methods: According to ISO-4823, a stainless-steel die was fabricated for this in vitro study. Three horizontal parallel lines, namely x, y, and z (25, 50, and 75µm in width and 25mm in length) running perpendicular to two vertical lines, namely D1 and D2 (0.075±0.008mm wide), were created on the superior surface of the die. Group A served as the control group with no disinfection. Disinfection was performed with 2% glutaraldehyde in group B, 5.25% sodium hypochlorite in group C, and ozone water in group D for 10 minutes. Totally, 60 samples were fabricated. The silicone impression samples were allowed to polymerize in a thermostatically controlled water bath at 35±1ºC for 10 minutes. The dimensional stability and accuracy of the silicone impression samples were evaluated by using a video measuring microscope and an optical microscope. Data were analyzed by two-way ANOVA followed by post-hoc analysis with the Scheffe test (alpha=0.05). Results: The highest dimensional stability (25.01mm) and accuracy (25.02µm) were seen in addition silicone putty and light body impression with ozone water disinfection, and the lowest parameters were seen in condensation silicon putty and light body disinfected with 2% glutaraldehyde (24.87mm and 24.88 µm, respectively); this difference was statistically significant (P<0.001). Conclusion: Ozone water disinfection caused minimal changes in dimensional stability and accuracy when compared to 2% glutaraldehyde and 5.25% sodium hypochlorite disinfection https://fid.tums.ac.ir/index.php/fid/article/view/4277