Tehran University of Medical Sciences Frontiers in Dentistry 2676-296X 11 3 2014 06 15 371 8 Hamid Razavian Assistant Professor of Endodontic, Torabinejad Dental Research Center and Department of Endodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran. Alireza Haerian Resident of Orthodontics, Torabinejad Dental Research Center and Department of Orthodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran. Hamid Mosleh Dentist, Dental Students' Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran. 2015 10 16 Many materials have been introduced for apexification each having their own advantages and disadvantages. This case report aims to present a new method of apexification using a combination of deproteinized bovine bone mineral (DBBM) and enamel matrix derivative (EMD). After irrigating the canal of the maxillary right canine with 2.5 % sodium hypochlorite, a mixture of Bio-Oss and EMD was packed into the apical region for formation of an apical barrier and the canal was obturated by thermoplastic gutta percha technique with AH26 sealer; coronal seal was achieved by resin bonded composite. The size of the periapical lesion decreased significantly after 3, 6, 12 and 18-months. The patient had no radiographic signs or clinical symptoms at 24-month follow up and complete maturation of the apex and healing of the periapical bone were achieved. https://fid.tums.ac.ir/index.php/fid/article/view/436 https://fid.tums.ac.ir/index.php/fid/article/download/436/412