Tehran University of Medical Sciences Frontiers in Dentistry 2676-296X 21 0 2024 09 23 1 11 Zahra Dadvar Department of Esthetic and Restorative Dentistry, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran Mohammad Esmaeel Ebrahimi Chaharom Department of Esthetic and Restorative Dentistry, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran Soodabeh Kimyai Department of Esthetic and Restorative Dentistry, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran Mahmoud Bahari Department of Esthetic and Restorative Dentistry, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran Mehdi Daneshpooy Department of Esthetic and Restorative Dentistry, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran 2022 05 16 2022 09 10 Objectives: The goal of this investigation was to see how a dentin pretreatment with 5% DMSO affected the microtensile bond strength (μTBS) of universal adhesives. Materials and Methods: In terms of adhesive kind and etching procedure, 32 healthy third human molars were randomly separated into eight groups. Three universal adhesives with etch-and-rinse and self-etch strategies (G-Premio Bond: GPB.ER/SE, All-Bond Universal: ABU.ER/SE, Prime & Bond Elect: PBE.ER/SE), one two-stage self-etch adhesive (Clearfil SE Bond: CSB), and one two-stage etch-and-rinse adhesive (Adper Single Bond 2: ASB) were employed in with and without DMSO modes (group/N=16). Dentin pretreatment was conducted with 50 μl of 5% DMSO, followed by the use of an adhesive. The μTBS of samples was tested. The influence of adhesive type and DMSO application on bond strength was evaluated using a two-way analysis of variance (ANOVA) (=0.05). Results: The dentin-adhesive μTBS was significantly affected by DMSO administration (P=0.003), type of adhesive (P=0.001), and the combination of DMSO application and type of adhesive (P=0.027). In the DMSO application mode, the average bond strength of universal adhesives with etch and rinse mode was significantly higher than in the non-application mode, but in the self-etch technique, there was no significant difference pattern between DMSO applications and non-application modes in terms of adhesive bond strength. Conclusion: The use of DMSO in an etch-and-rinse technique can dramatically enhance the universal adhesive-dentin μTBS and has promise benefits for clinicians in terms of enhancing dentin bond performance.  https://fid.tums.ac.ir/index.php/fid/article/view/4477