Tehran University of Medical Sciences Frontiers in Dentistry 2676-296X 11 2 2014 04 15 225 32 Sara Tavassoli-Hojjati Assistant Professor, Department of pediatric Dentistry, Dental school, Shahed University, Tehran, Iran. Elham Aliasghar Dentist, Department of pediatric Dentistry, Dental school, Shahed University, Tehran, Iran. Fatemeh Ahmadian Babaki Assistant Professor, Department of Pharmacology and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Fatemeh Emadi Assistant Professor, Department of Pharmacology and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Maliheh Parsa PhD Student, Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Shohreh Tavajohi General Technician, Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Maryam Ahmadyar Dentist, Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Seyed Nasser Ostad Proffesor, Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 16 There is evidence indicating that pomegranate juice contains many of the essential properties necessary to retain cell viability and cell proliferation. These properties indicate that pomegranate juice is a suitable storage medium for avulsed teeth. However, this idea has not yet been tested. In this study, the capacity of pomegranate juice (PJ) as a storage medium for retaining avulsed teeth was evaluated.PDL fibroblasts were obtained from healthy human premolars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). Cultured cells were subjected to different concentrations of pomegranate juice (PJ), 1% Hank's balanced salt solution (HBSS) and tap water for 1, 3, 6 and 24 hours. PDL cell viability was assessed by the neutral red uptake assay.The results indicated that 7.5% PJ was the most effective solution for maintaining PDL cell viability amongst all the experimental solution's and time intervals (P https://fid.tums.ac.ir/index.php/fid/article/view/513 https://fid.tums.ac.ir/index.php/fid/article/download/513/489