Tehran University of Medical Sciences Frontiers in Dentistry 2676-296X 23 0 2026 10 10 5537 5537 EN Sanaz Mihandoust Dental Sciences Research Center, School of Dentistry, Guilan University of Medical Sciences, Rasht, Iran Ali Seyed-Monir Private Practice, Rasht, Iran Mehrsima Ghavami-Lahiji 1. Dental Sciences Research Center, School of Dentistry, Guilan University of Medical Sciences, Rasht, Iran 3. Department of Restorative Dentistry, School of Dentistry, Guilan University of Medical Sciences, Rasht, Iran Keyvan Moharamzadeh Hamdan Bin Mohammed College of Dental Medicine (HBMCDM), Mohammed Bin Rashid University of Medicine and Health Sciences (MBRU), Dubai, United Arab Emirates Lobat Tayebi Institute for Engineering in Medicine, Health, & Human Performance (EnMed), Batten College of Engineering and Technology, Old Dominion University, Norfolk, VA, 23508, USA 2025 02 23 2025 11 03 Developing a laboratory-grown construct integrating viable bone and oral mucosal tissues with strong adhesion between the hard and soft tissue layers presents a significant challenge. This engineered tissue construct holds promise as a versatile graft material for diverse oral surgeries and as an in vitro model for evaluating dental materials, dental implants, and oral healthcare products, potentially replacing the need for in vivo studies. Additionally, it offers a valuable tool for investigating various aspects of oral diseases and evaluating the efficacy of different treatments, eliminating the need for animal models. The success of such osteo-mucosal constructs depends on achieving proper adhesion between the bone and connective tissue layers, prompting researchers to conduct studies exploring interlayer adhesion mechanisms. This review provides a comprehensive overview of advances in osteo-mucosal models, conducted through a search of articles published in journals indexed in PubMed, ScienceDirect, Scopus, and Google Scholar databases from January 2010 to January 2024. First, we outline the methods used to construct various components of osteo-mucosal models, including the materials and fabrication techniques. Next, we provide a detailed discussion of the adhesion methods employed. Lastly, we compare the effectiveness of each adhesion method by summarizing the results of cell viability studies, which are crucial for the success of tissue regeneration. This review provides a comprehensive understanding of recent advancements in osteo-mucosal models and their interfacial adhesion, paving the way for promising clinical applications. https://fid.tums.ac.ir/index.php/fid/article/view/5537 https://fid.tums.ac.ir/index.php/fid/article/download/5537/2246