Tehran University of Medical Sciences Frontiers in Dentistry 2676-296X 10 6 2013 12 15 540 7 Noushin Shokouhinejad Associate Professor, Dental Research Center Dentistry Research Institute, Department of Endodontics, School of Dentistry Tehran University of Medical Sciences, Tehran, Iran ; Iranian Center for Endodontic Research, Tehran, Iran. Hasan Razmi Associate Professor, Dental Research Center Dentistry Research Institute, Department of Endodontics, School of Dentistry Tehran University of Medical Sciences, Tehran, Iran ; Iranian Center for Endodontic Research, Tehran, Iran. Mohammad Hossein Nekoofar Assistant Professor, Dental Research Center, Department of Endodontics, School of Dentistry Tehran University of Medical Sciences, Tehran, Iran ; Endodontology Research Group, School of Dentistry, Cardiff University, Heath Park, Cardiff, UK. Sepideh Sajadi Assistant Professor, School of Dentistry, Arak University of Medical Sciences, Arak, Iran. Paul Mh Dummer Endodontology Research Group, School of Dentistry, Cardiff University, Heath Park, Cardiff, UK. Mehrfam Khoshkhounejad Assistant Professor, Dental Research Center, Department of Endodontics, School of Dentistry Tehran University of Medical Sciences, Tehran, Iran. 2015 10 16 This study compared the push-out bond strength of EndoSequence Root Repair Material (ERRM) and Bioaggregate (BA), new bioceramic materials, to that of mineral trioxide aggregate (MTA) after incubation in phosphate-buffered saline (PBS), a synthetic tissue fluid, for either 1 week or 2 months.One-hundred and twenty root sections were filled with ProRoot MTA, BA, or ERRM. Each tested material was then randomly divided into two subgroups (n = 20): root sections were immersed in PBS for 1 week or 2 months. The bond strengths were measured using a universal testing machine. After that, the failure modes were examined with stereomicroscopy and scanning electron microscopy (SEM). The push-out data and failure mode categories were analyzed by two-way ANOVA and chi-square tests, respectively.The bond strength of ERRM was significantly higher than that of BA and MTA at both incubation periods. No significant difference was found between the bond strength of MTA and BA at either 1 week or 2 months. Increasing the incubation time to 2 months resulted in a significant increase in bond strength of all the materials. The failure mode was mainly mixed for MTA and BA, but cohesive for ERRM at both incubation periods.ERRM had significantly higher bond strength to root canal walls compared to MTA and BA. Increasing the incubation time significantly improved the bond strength and bioactive reaction products of all materials. https://fid.tums.ac.ir/index.php/fid/article/view/583 https://fid.tums.ac.ir/index.php/fid/article/download/583/558