Original Article

Evaluation of Degree of Conversion, Resin-Dentin Bond Strength, and Durability of Polydopamine Incorporated Total Etch Adhesive System

Abstract

Objectives: This study aimed to evaluate the degree of conversion (DC%), shear bond strength (SBS), bond durability, and the resin-dentin interface of polydopamine (PDA) incorporated total-etch adhesive system.
Materials and Methods: Five percentage by weight (wt%) concentration of PDA incorporated adhesive was prepared and its polymerization was evaluated using Fourier-transform infrared spectroscopy. The results were compared with the DC% of conventional total-etch adhesive (Adper Single Bond 2) using independent t-test. Occlusal surfaces of 80 freshly extracted human premolars were sectioned to expose the dentin, which was acid-etched. The samples were divided into two groups (n=40) of total-etch adhesive and total-etch adhesive+PDA. Composite resin blocks were built up over the bonded surface and cured. Each group was subdivided into two subgroups (n=20) of immediate and post-aging evaluation. Samples were immersed in 10% sodium hypochlorite for five hours, and then, the SBS was evaluated using a universal testing machine at a crosshead speed of 1 mm/minute. The resin-dentin interface was evaluated using confocal laser scanning microscopy. Data were analyzed using two-way analysis of variance and post-hoc Bonferroni test.
Results: DC% was not affected by the addition of 5% PDA to the adhesive (group 2). The SBS of group 2A was significantly higher than that of group 1A. There was no significant reduction of SBS in group 2B. The hybrid layer was less degraded after aging in group 2B compared to group 2A.
Conclusions: PDA incorporated adhesive increased the immediate bond strength and durability without changing the DC%.

1. Amaral FL, Colucci V, Palma-Dibb RG, Corona SA. Assessment of in vitro methods used to promote adhesive interface degradation: a critical review. J Esthet Restor Dent. 2007;19(6):340-53; discussion 354.
2. Armstrong SR, Vargas MA, Chung I, Pashley DH, Campbell JA, Laffoon JE, et al. Resin-dentin interfacial ultrastructure and microtensile dentin bond strength after five-year water storage. Oper Dent. 2004 Nov-Dec;29(6):705-12.
3. Cardoso MV, de Almeida Neves A, Mine A, Coutinho E, Van Landuyt K, De Munck J, et al. Current aspects on bonding effectiveness and stability in adhesive dentistry. Aust Dent J. 2011 Jun;56 Suppl 1:31-44.
4. Shono Y, Terashita M, Shimada J, Kozono Y, Carvalho RM, Russell CM, et al. Durability of resin-dentin bonds. J Adhes Dent. 1999 Autumn;1(3):211-8.
5. Hashimoto M, Ohno H, Kaga M, Endo K, Sano H, Oguchi H. In vivo degradation of resin-dentin bonds in humans over 1 to 3 years. J Dent Res. 2000 Jun;79(6):1385-91.
6. Liu Y, Tjäderhane L, Breschi L, Mazzoni A, Li N, Mao J, et al. Limitations in bonding to dentin and experimental strategies to prevent bond degradation. J Dent Res. 2011 Aug;90(8):953-68.
7. Wang Y, Spencer P. Quantifying adhesive penetration in adhesive/dentin interface using confocal Raman microspectroscopy. J Biomed Mater Res. 2002 Jan;59(1):46-55.
8. Wang Y, Spencer P. Hybridization efficiency of the adhesive/dentin interface with wet bonding. J Dent Res. 2003 Feb;82(2):141-5.
9. Mazzoni A, Pashley DH, Nishitani Y, Breschi L, Mannello F, Tjäderhane L, et al. Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives. Biomaterials. 2006 Sep;27(25):4470-6.
10. De Munck J, Van den Steen PE, Mine A, Van Landuyt KL, Poitevin A, Opdenakker G, et al. Inhibition of enzymatic degradation of adhesive-dentin interfaces. J Dent Res. 2009 Dec;88(12):1101-6.
11. Mahalaxmi S, Madhubala MM, Jayaraman M, Sathyakumar S. Evaluation of matrix metalloproteinase and cysteine cathepsin activity in dentin hybrid layer by gelatin zymography. Indian J Dent Res. 2016 Nov-Dec;27(6):652-656.
12. Tanaka J, Ishikawa K, Yatani H, Yamashita A, Suzuki K. Correlation of dentin bond durability with water absorption of bonding layer. Dent Mater J. 1999 Mar;18(1):11-8.
13. Ito S, Saito T, Tay FR, Carvalho RM, Yoshiyama M, Pashley DH. Water content and apparent stiffness of non-caries versus caries-affected human dentin. J Biomed Mater Res B Appl Biomater. 2005 Jan 15;72(1):109-16.
14. Lee BP, Messersmith PB, Israelachvili JN, Waite JH. Mussel-Inspired Adhesives and Coatings. Annu Rev Mater Res. 2011 Aug 1;41:99-132.
15. Ho CC, Ding SJ. Structure, properties and applications of mussel-inspired polydopamine. J Biomed Nanotechnol. 2014 Oct;10(10):3063-84.
16. Dalsin JL, Hu BH, Lee BP, Messersmith PB. Mussel adhesive protein mimetic polymers for the preparation of nonfouling surfaces. J Am Chem Soc. 2003 Apr 9;125(14):4253-8.
17. Jo SH, Sohn JS. Biomimetic adhesive materials containing cyanoacryl group for medical application. Molecules. 2014 Oct 17;19(10):16779-93.
18. Lee SB, González-Cabezas C, Kim KM, Kim KN, Kuroda K. Catechol-Functionalized Synthetic Polymer as a Dental Adhesive to Contaminated Dentin Surface for a Composite Restoration. Biomacromolecules. 2015 Jul;16(8):2265-75.
19. Zhou YZ, Cao Y, Liu W, Chu CH, Li QL. Polydopamine-induced tooth remineralization. ACS Appl Mater Interfaces. 2012 Nov;4(12):6901-10.
20. Xu Q, Li Q, Chen J, Zhang W, Wu X, Cao Y. [Effect of dopamine on the activity of matrix metalloproteinases and degradation of dentin collagen]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2015 Mar;50(3):186-9.
21. Toledano M, Osorio R, Albaladejo A, Aguilera FS, Osorio E. Differential effect of in vitro degradation on resin-dentin bonds produced by self-etch versus total-etch adhesives. J Biomed Mater Res A. 2006 Apr;77(1):128-35.
22. Lee H, Scherer NF, Messersmith PB. Single-molecule mechanics of mussel adhesion. Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):12999-3003.
23. Yazdi FM, Moosavi H, Atai M, Zeynali M. Dentin bond strength and degree of conversion evaluation of experimental self-etch adhesive systems. J Clin Exp Dent. 2015 Apr 1;7(2):e243-9.
24. Lee BP, Chao CY, Nunalee FN, Motan E, Shull KR, Messersmith PB. Rapid gel formation and adhesion in photocurable and biodegradable block copolymers with high DOPA content. Macromolecules. 2006 Feb;39(5):1740-8.
25. Nofsinger JB, Forest SE, Eibest LM, Gold KA, Simon JD. Probing the building blocks of eumelanins using scanning electron microscopy. Pigment Cell Res. 2000 Jun;13(3):179-84.
26. Mehdizadeh M, Weng H, Gyawali D, Tang L, Yang J. Injectable citrate-based mussel-inspired tissue bioadhesives with high wet strength for sutureless wound closure. Biomaterials. 2012 Nov;33(32):7972-83.
27. Garbui BU, Botta SB, Reis AF, Matos AB. Comparison of chemical aging and water immersion time on durability of resin-dentin interface produced by an etch-and-rinse adhesive. J Contemp Dent Pract. 2012 Jul 1;13(4):464-71.
28. Waite JH, Qin X. Polyphosphoprotein from the adhesive pads of Mytilus edulis. Biochemistry. 2001 Mar 6;40(9):2887-93.
29. Burzio LA, Waite JH. Cross-linking in adhesive quinoproteins: studies with model decapeptides. Biochemistry. 2000 Sep 12;39(36):11147-53.
30. Lee H, Dellatore SM, Miller WM, Messersmith PB. Mussel-inspired surface chemistry for multifunctional coatings. Science. 2007 Oct 19;318(5849):426-30.
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IssueVol 17 (Continuously Published Article-Based) QRcode
SectionOriginal Article
DOI https://doi.org/10.18502/fid.v17i35.5199
Keywords
Polydopamine 3M Single Bond Dental Adhesive Dental Etching Polymerization Dental Bonding

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How to Cite
1.
James V, Madhubala M, Devarajan S, Mahalaxmi S, Sathyakumar S. Evaluation of Degree of Conversion, Resin-Dentin Bond Strength, and Durability of Polydopamine Incorporated Total Etch Adhesive System. Front Dent. 2021;17.