Effect of Different Cleansing Protocols on Bond Strength of Composite Resin to Dentin Contaminated with Hemostatic Agent: An In Vitro Study
,
Abstract
Objectives: Inadequate removal of the hemostatic agent can adversely affect the bond strength of restorations to the tooth structure. This study aimed to assess the effect of different cleansing protocols on the shear bond strength (SBS) of an etch-and-rinse adhesive to dentin contaminated with aluminum chloride hemostatic agent.
Materials and Methods: In this experimental study, the mid-coronal dentin of 96 premolars was exposed. They were contaminated with a hemostatic agent (ViscoStat Clear) and then randomly divided into 7 groups (n=12). One group served as the control. The groups underwent various cleaning methods as follows: water spray, aluminum oxide particles (27µ diameter), a slurry of pumice with water, GC dentin conditioner (GCDC), sodium hypochlorite 2% (SHC), and chlorhexidine 2% (CHX). Composite cylinders were then fabricated and bonded to the surfaces using Scotchbond Multi-Purpose etch-and-rinse bonding agent. After thermocycling (10,000 cycles), the SBS was measured using a universal testing machine. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey HSD (honestly significant difference) test, and the significance level was set at 0.05.
Results: The SBS of the groups was significantly different (P=0.036). The SBS was the highest in the CHX and SHC groups, and the lowest SBS was related to the control group and GCDC groups. The difference between other groups was not significant (P=0.996).
Conclusion: CHX and SHC yielded the highest bond strength among the tested modalities for cleansing the ViscoStat Clear from the tooth surface.
1. Bernades Kde O, Hilgert LA, Ribeiro AP, Garcia FC, Pereira PN. The influence of hemostatic agents on dentin and enamel surfaces and dental bonding: a systematic review. J Am Dent Assoc. 2014 Nov;145(11):1120-8.
2. Pucci CR, Araújo RM, Lacerda AJ, Souza MA, Huhtala MF, Feitosa FA. Effects of Contamination by Hemostatic Agents and Use of Cleaning Agent on Etch-and-Rinse Dentin Bond Strength. Braz Dent J. 2016 Dec;27(6):688-92.
3. Ajami AA, Kahnamoii MA, Kimyai S, Oskoee SS, Pournaghi-Azar F, Bahari M, et al. Effect of three different contamination removal methods on bond strength of a self-etching adhesive to dentin contaminated with an aluminum chloride hemostatic agent .J Contemp Dent Pract. 2013 Jan 1;14(1):26.
4. Chaiyabutr Y, Kois JC. The effect of tooth-preparation cleansing protocol on the bond strength of self-adhesive resin cement to dentin contaminated with a hemostatic agent. Oper Dent. 2011 Jan-Feb;36(1):18-26.
5. Tuncer D, Basaran S, Halacoglu DM, Yamanel K, Celik C, Arhun N. Effect of hemostatic agent application on the shear bond strength of contemporary/multi-mode adhesive systems. Oral Health Dent Manag. 2014 Mar;13(1):103-6.
6. Chaiyabutr Y, Kois JC. The effect of tooth-preparation cleansing protocol on the bond strength of self-adhesive resin cement to dentin contaminated with a hemostatic agent. Oper Dent. 2011 Jan-Feb;36(1):18-26.
7. Arslan S, ERTAS H, Zorba YO. Influence of Ankaferd Blood Stopper on shear bond strength of bonding systems. Dent Mater. 2012 Mar;31(2):226-31.
8. Araújo IS, Prado CJD, Raposo LHA, Soares CJ, Zanatta RF, Torres CRG, et al. Influence of Hemostatic Solution on Bond Strength and Physicochemical Properties of Resin Cement. Braz Dent J. 2017 Sep-Oct;28(5):624-631.
9. Trakyali G, Oztoprak MO. Plant extract ankaferd blood stopper effect on bond strength. Angle Orthod. 2010 May;80(3):570-4.
10. Khoroushi M, Hosseini-Shirazi M, Farahbod F, Keshani F. Composite resin bond strength to caries-affected dentin contaminated with 3 different hemostatic agents. Gen Dent. 2016 Jul-Aug;64(4):e11-5.
11. Moosavi H, Mohammadipour HS, Ghavamnasiri M, Alizadeh S. Effect of Bleaching and Thermocycling on Resin-Enamel Bond Strength. Int J Biomater. 2015;2015:921425.
12. Gray GB, Carey GP, Jagger DC. An in vitro investigation of a comparison of bond strengths of composite to etched and air-abraded human enamel surfaces. J Prosthodont. 2006 Jan-Feb;15(1):2-8.
13. Güngör AY, Alkis H, Turkkahraman H. Effects of contamination by either blood or a hemostatic agent on the shear bond strength of orthodontic buttons. Korean J Orthod. 2013 Apr;43(2):96-100.
14. Tarighi P, Khoroushi M. A review on common chemical hemostatic agents in restorative dentistry. Dent Res J (Isfahan). 2014;11(4):423-8.
15. Nouri S, Sharif MR, Panahi Y, Ghanei M, Jamali B. Efficacy and safety of aluminum chloride in controlling external hemorrhage: an animal model study. Iran Red Crescent Med J. 2015 Mar 20;17(3):e19714.
16. Xu X, Chen Q, Lederer A, Bernau C, Lai G, Kaisarly D, Dent DM. Shear bond strength of two adhesives to bovine dentin contaminated with various astringents. Am J Dent. 2015 Aug;28(4):229-34.
17. Ebrahimi SF, Shadman N, Abrishami A. Effect of ferric sulfate contamination on the bonding effectiveness of etch-and-rinse and self-etch adhesives to superficial dentin. J Conserv Dent. 2013 Mar;16(2):126-30.
18. Sahin O, Dede DÖ, Köroğlu A, Özgüven Y, Doğan DÖ. Effect of irradiation on the shear bond strength of self-adhesive luting cement in different preparation depths. Niger J Clin Pract. 2017 Aug;20(8):924-929.
19. Holderegger C, Sailer I, Schuhmacher C, Schläpfer R, Hämmerle C, Fischer J. Shear bond strength of resin cements to human dentin. Dent Mater. 2008 Jul;24(7):944-50.
20. Sharafeddin F, Farhadpour H. Evaluation of Shear Bond Strength of Total- and Self-etching Adhesive Systems after Application of Chlorhexidine to Dentin Contaminated with a Hemostatic Agent. J Dent (Shiraz). 2015 Sep;16(3):175-81.
21. Alaghehmad H, Mansouri E, Esmaili B, Bijani A, Nejadkarimi S, Rahchamani M. Effect of 0.12% chlorhexidine and zinc nanoparticles on the microshear bond strength of dentin with a fifth-generation adhesive. Eur J Dent. 2018 Jan-Mar;12(1):105-110.
22. Flury S, Peutzfeldt A, Schmidlin PR, Lussi A. Exposed Dentin: Influence of Cleaning Procedures and Simulated Pulpal Pressure on Bond Strength of a Universal Adhesive System. PLoS One. 2017 Jan 12;12(1):e0169680.
23. Campos EA, Correr GM, Leonardi DP, Pizzatto E, Morais EC. Influence of chlorhexidine concentration on microtensile bond strength of contemporary adhesive systems. Braz Oral Res. 2009 Jul-Sep;23(3):340-5.
24. Del Carpio-Perochena A, Monteiro Bramante C, Hungaro Duarte M, Bombarda de Andrade F, Zardin Graeff M, et al. Effect of Temperature, Concentration and Contact Time of Sodium Hypochlorite on the Treatment and Revitalization of Oral Biofilms. J Dent Res Dent Clin Dent Prospects. 2015 Fall;9(4):209-15.
25. Taneja S, Mishra N, Malik S. Comparative evaluation of human pulp tissue dissolution by different concentrations of chlorine dioxide, calcium hypochlorite and sodium hypochlorite: An in vitro study. J Conserv Dent. 2014 Nov;17(6):541-5.
26. Abuhaimed TS, Abou Neel EA. Sodium Hypochlorite Irrigation and Its Effect on Bond Strength to Dentin. Biomed Res Int. 2017;2017:1930360.
27. Ryou H, Turco G, Breschi L, Tay FR, Pashley DH, Arola D. On the stiffness of demineralized dentin matrices. Dent Mater. 2016 Feb;32(2):161-70.
28. Favarão J, Zanini MM, Coelho Sinhoreti MA, Flores Abuna G, Girelli Júnior C. Immediate and 6-month Bond Strengths of Different Adhesives in the Oral Environment. J Adhes Dent. 2017 Dec 18:475-481.
2. Pucci CR, Araújo RM, Lacerda AJ, Souza MA, Huhtala MF, Feitosa FA. Effects of Contamination by Hemostatic Agents and Use of Cleaning Agent on Etch-and-Rinse Dentin Bond Strength. Braz Dent J. 2016 Dec;27(6):688-92.
3. Ajami AA, Kahnamoii MA, Kimyai S, Oskoee SS, Pournaghi-Azar F, Bahari M, et al. Effect of three different contamination removal methods on bond strength of a self-etching adhesive to dentin contaminated with an aluminum chloride hemostatic agent .J Contemp Dent Pract. 2013 Jan 1;14(1):26.
4. Chaiyabutr Y, Kois JC. The effect of tooth-preparation cleansing protocol on the bond strength of self-adhesive resin cement to dentin contaminated with a hemostatic agent. Oper Dent. 2011 Jan-Feb;36(1):18-26.
5. Tuncer D, Basaran S, Halacoglu DM, Yamanel K, Celik C, Arhun N. Effect of hemostatic agent application on the shear bond strength of contemporary/multi-mode adhesive systems. Oral Health Dent Manag. 2014 Mar;13(1):103-6.
6. Chaiyabutr Y, Kois JC. The effect of tooth-preparation cleansing protocol on the bond strength of self-adhesive resin cement to dentin contaminated with a hemostatic agent. Oper Dent. 2011 Jan-Feb;36(1):18-26.
7. Arslan S, ERTAS H, Zorba YO. Influence of Ankaferd Blood Stopper on shear bond strength of bonding systems. Dent Mater. 2012 Mar;31(2):226-31.
8. Araújo IS, Prado CJD, Raposo LHA, Soares CJ, Zanatta RF, Torres CRG, et al. Influence of Hemostatic Solution on Bond Strength and Physicochemical Properties of Resin Cement. Braz Dent J. 2017 Sep-Oct;28(5):624-631.
9. Trakyali G, Oztoprak MO. Plant extract ankaferd blood stopper effect on bond strength. Angle Orthod. 2010 May;80(3):570-4.
10. Khoroushi M, Hosseini-Shirazi M, Farahbod F, Keshani F. Composite resin bond strength to caries-affected dentin contaminated with 3 different hemostatic agents. Gen Dent. 2016 Jul-Aug;64(4):e11-5.
11. Moosavi H, Mohammadipour HS, Ghavamnasiri M, Alizadeh S. Effect of Bleaching and Thermocycling on Resin-Enamel Bond Strength. Int J Biomater. 2015;2015:921425.
12. Gray GB, Carey GP, Jagger DC. An in vitro investigation of a comparison of bond strengths of composite to etched and air-abraded human enamel surfaces. J Prosthodont. 2006 Jan-Feb;15(1):2-8.
13. Güngör AY, Alkis H, Turkkahraman H. Effects of contamination by either blood or a hemostatic agent on the shear bond strength of orthodontic buttons. Korean J Orthod. 2013 Apr;43(2):96-100.
14. Tarighi P, Khoroushi M. A review on common chemical hemostatic agents in restorative dentistry. Dent Res J (Isfahan). 2014;11(4):423-8.
15. Nouri S, Sharif MR, Panahi Y, Ghanei M, Jamali B. Efficacy and safety of aluminum chloride in controlling external hemorrhage: an animal model study. Iran Red Crescent Med J. 2015 Mar 20;17(3):e19714.
16. Xu X, Chen Q, Lederer A, Bernau C, Lai G, Kaisarly D, Dent DM. Shear bond strength of two adhesives to bovine dentin contaminated with various astringents. Am J Dent. 2015 Aug;28(4):229-34.
17. Ebrahimi SF, Shadman N, Abrishami A. Effect of ferric sulfate contamination on the bonding effectiveness of etch-and-rinse and self-etch adhesives to superficial dentin. J Conserv Dent. 2013 Mar;16(2):126-30.
18. Sahin O, Dede DÖ, Köroğlu A, Özgüven Y, Doğan DÖ. Effect of irradiation on the shear bond strength of self-adhesive luting cement in different preparation depths. Niger J Clin Pract. 2017 Aug;20(8):924-929.
19. Holderegger C, Sailer I, Schuhmacher C, Schläpfer R, Hämmerle C, Fischer J. Shear bond strength of resin cements to human dentin. Dent Mater. 2008 Jul;24(7):944-50.
20. Sharafeddin F, Farhadpour H. Evaluation of Shear Bond Strength of Total- and Self-etching Adhesive Systems after Application of Chlorhexidine to Dentin Contaminated with a Hemostatic Agent. J Dent (Shiraz). 2015 Sep;16(3):175-81.
21. Alaghehmad H, Mansouri E, Esmaili B, Bijani A, Nejadkarimi S, Rahchamani M. Effect of 0.12% chlorhexidine and zinc nanoparticles on the microshear bond strength of dentin with a fifth-generation adhesive. Eur J Dent. 2018 Jan-Mar;12(1):105-110.
22. Flury S, Peutzfeldt A, Schmidlin PR, Lussi A. Exposed Dentin: Influence of Cleaning Procedures and Simulated Pulpal Pressure on Bond Strength of a Universal Adhesive System. PLoS One. 2017 Jan 12;12(1):e0169680.
23. Campos EA, Correr GM, Leonardi DP, Pizzatto E, Morais EC. Influence of chlorhexidine concentration on microtensile bond strength of contemporary adhesive systems. Braz Oral Res. 2009 Jul-Sep;23(3):340-5.
24. Del Carpio-Perochena A, Monteiro Bramante C, Hungaro Duarte M, Bombarda de Andrade F, Zardin Graeff M, et al. Effect of Temperature, Concentration and Contact Time of Sodium Hypochlorite on the Treatment and Revitalization of Oral Biofilms. J Dent Res Dent Clin Dent Prospects. 2015 Fall;9(4):209-15.
25. Taneja S, Mishra N, Malik S. Comparative evaluation of human pulp tissue dissolution by different concentrations of chlorine dioxide, calcium hypochlorite and sodium hypochlorite: An in vitro study. J Conserv Dent. 2014 Nov;17(6):541-5.
26. Abuhaimed TS, Abou Neel EA. Sodium Hypochlorite Irrigation and Its Effect on Bond Strength to Dentin. Biomed Res Int. 2017;2017:1930360.
27. Ryou H, Turco G, Breschi L, Tay FR, Pashley DH, Arola D. On the stiffness of demineralized dentin matrices. Dent Mater. 2016 Feb;32(2):161-70.
28. Favarão J, Zanini MM, Coelho Sinhoreti MA, Flores Abuna G, Girelli Júnior C. Immediate and 6-month Bond Strengths of Different Adhesives in the Oral Environment. J Adhes Dent. 2017 Dec 18:475-481.
| Files | ||
| Issue | Vol 17 (Continuously Published Article-Based) | |
| Section | Original Article | |
| DOI | https://doi.org/10.18502/fid.v17i31.4861 | |
| Keywords | ||
| Adhesives Aluminum Chloride Dental Bonding Hemostatics | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Saati K, Tabatabaei SF, Etemadian D, Sadaghiani M. Effect of Different Cleansing Protocols on Bond Strength of Composite Resin to Dentin Contaminated with Hemostatic Agent: An In Vitro Study. Front Dent. 2020;17.
