Original Article

Effect of Contamination with Hemostatic Agent on Shear Bond Strength of Composite to Dentin Using G-Premio and Single Bond Universal Adhesives


Objectives: Bond strength of composite restorations plays an important role in their success. This in vitro study evaluated the effect of a hemostatic agent on shear bond strength of universal adhesives.

Materials and Methods: Thirty-six extracted human molars were used in this study. Buccal and lingual surfaces were reduced to obtain flat dentin surfaces and were ground with a silicone paper. The samples were randomly divided into three groups (n=12) based on the application of hemostatic agent: group 1: no contamination (control), group 2: aluminum chloride application, and group 3: ferric sulfate application. Each group was then divided into two subgroups (n=6) for using G-Premio and Single Bond Universal. Resin cylinders (Filtek Z550) were bonded to dentin surfaces according to the manufacturer’s instructions. After 1000 thermal cycles, shear load was applied to the specimens using a universal testing machine at a crosshead speed of 1 mm/min until failure. Data were statistically analyzed using two-way ANOVA and Tukey’s post-hoc test (α=0.05).

Results: There were statistically significant differences in shear bond strength of the three main groups for both G-Premio and Single Bond Universal (P<0.05). When the adhesive systems were compared with each other, G-Premio showed higher shear bond strength than Single Bond Universal (P<0.05).

Conclusion: Contamination with hemostatic agents had an adverse effect on the shear bond strength of universal adhesives. Moreover, G-Premio yielded a higher bond strength than Single Bond Universal.

Keywords: Hemostatics; Shear Strength; Adhesives

Koppolu M, Gogala D, Mathew VB, Thangala V, Deepthi M, Sasidhar N. Effect of saliva and blood contamination on the bond strength of self-etching adhesive system-An in vitro study. J Conserv Dent. 2012 Jul;15(3):270.

Sharafeddin F, Moradian H. Microleakage of class II combined Amalgam-Composite restorations using different composites and bonding agents. Front Dent. 2008;5(3):126-30.

Sharafeddin F, Yousefi H, Modiri S, Tondari A, Jahromi SS. Microleakage of posterior composite restorations with fiber inserts using two adhesives after aging. J Dent. 2013 Sep;14(3):90.

Gupta G, Kumar S, Rao H, Garg P, Kumar R, Sharma A, et al. Astringents in dentistry: a review. Asian J Pharm Health Sci. 2012;2(3):428-32.

Harnirattisai C, Kuphasuk W, Senawongse P, Tagami J. Bond strengths of resin cements to astringent-contaminated dentin. Oper Dent. 2009;34(4):415-22.

Nowakowska D, Saczko J, Kulbacka J, Choromanska A, Raszewski Z. Cytotoxic potential of vasoconstrictor experimental gingival retraction agents-in vitro study on primary human gingival fibroblasts. Folia biologica. 2012;58(1):37.

Felpel LP. A review of pharmacotherapeutics for prosthetic dentistry: Part I. J Prosthet Dent. 1997 Mar;77(3):285-92.

Vickers FJ, Baumgartner JC, Marshall G. Hemostatic efficacy and cardiovascular effects of agents used during endodontic surgery. J Endod. 2002 Apr;28(4):322-3.

Azargoon H, Williams BJ, Solomon ES, Kessler HP, He J, Spears R. Assessment of hemostatic efficacy and osseous wound healing using HemCon dental dressing. J Endod. 2011 Jun;37(6):807-11.

Fischer DE. Tissue management: a new solution to an old problem. Gen Dent. 1987 May-Jun;35(3):178-82.

Lemon RR, Steele PJ, Jeansonne BG. Ferric sulfate hemostasis: effect on osseous wound healing. I. Left in situ for maximum exposure. J Endod. 1993 Apr;19(4):170-3.

Ebrahimi SF, Shadman N, Abrishami A. Effect of ferric sulfate contamination on the bonding effectiveness of etch-and-rinse and self-etch adhesives to superficial dentin. J Conserv Dent. 2013 Mar;16(2):126.

Dey S, Shenoy A, Kundapur SS, Das M, Gunwal M, Bhattacharya R. Evaluation of the effect of different contaminants on the shear bond strength of a two-step self-etch adhesive system, one-step, self-etch adhesive system and a total-etch adhesive system. J Int Oral Health. 2016;8(3):378.

Kuphasuk W, Harnirattisai C, Senawongse P, Tagami J. Bond strengths of two adhesive systems to dentin contaminated with a hemostatic agent. Oper Dent. 2007 Jul-Aug;32(4):399-405.

Tuncer D, Basaran S, Halaçoglu DM, Yamanel K, Çelik Ç, Arhun N. Effect of haemostatic agent application on the shear bond strength of contemporary/multi-mode adhesive systems. Oral Health Dent Manag. 2014 Mar;13(1):103-6.

Sharafeddin F, Farhadpour H. Evaluation of shear bond strength of total-and self-etching adhesive systems after application of chlorhexidine to dentin contaminated with a hemostatic agent. J Dent (Shiraz). 2015 Sep;16(3):175-81.

Bogra P, Gupta S, Kumar S. Comparative evaluation of microleakage in class II cavities restored with Ceram X and Filtek P-90: An in vitro study. Contemp Clin Dent. 2012 Jan;3(1):9-14.

Braga RR, Meira JB, Boaro LC, Xavier T. Adhesion to tooth structure: a critical review of “macro” test methods. Dent Mater. 2010 Feb;26(2):e38-49.

de Carvalho Mendonça EC, Vieira SN, Kawaguchi FA, Powers J, Matos A. Influence of blood contamination on bond strength of a self-etching system. Eur J Dent. 2010 Jul;4(3):280-6.

Araújo IS, Prado CJ, Raposo LH, Soares CJ, Zanatta RF, Torres CR, et al. Influence of Hemostatic Solution on Bond Strength and Physicochemical Properties of Resin Cement. Braz Dent J. 2017 Sep;28(5):624-31.

Tarighi P, Khoroushi M. A review on common chemical hemostatic agents in restorative dentistry. Dent Res J (Isfahan). 2014 Jul;11(4):423-8.

Shadman N, Ebrahimi SF, Mollaie N. Sealing of adhesive systems in ferric sulfate-contaminated dentinal margins in class V composite resin restorations. Dent Res Dent Clin Dent Prospect. Winter 2016;10(1):17-22.

O'Keefe KL, Pinzon LM, Rivera B, Powers JM. Bond strength of composite to astringent-contaminated dentin using self-etching adhesives. Am J Dent. 2005 Jun;18(3):168-72.

Sofan E, Sofan A, Palaia G, Tenore G, Romeo U, Migliau G. Classification review of dental adhesive systems: from the IV generation to the universal type. Ann Stomatol (Roma). 2017 Jul 3;8(1):1-17.

Perdigão J. Dentin bonding—Variables related to the clinical situation and the substrate treatment. Dent Mater. 2010 Feb;26(2):e24-37.

Tay F, Gwinnett J, Wei SH. Relation between water content in acetone/alcohol-based primer and interfacial ultrastructure. J Dent. 1998 Mar;26(2):147-56.

Schüpbach P, Krejci I, Lutz F. Dentin bonding: effect of tubule orientation on hybrid‐layer formation. Eur J Oral Sci. 1997 Aug;105(4):344-52.

Yoshida Y, Yoshihara K, Hayakawa S, Nagaoka N, Okihara T, Matsumoto T, et al. HEMA inhibits interfacial nano-layering of the functional monomer MDP. J Dent Res. 2012 Nov;91(11):1060-5.

Jang JH, Jeon BK, Mo SY, Park M, Choi D, Choi KK, et al. Effect of various agitation methods on adhesive layer formation of HEMA-free universal dentin adhesive. Dent Mater J. 2019 Feb;38(1):101-106.

Reis A, Pellizzaro A, Dal-Bianco K, Gomes OM, Patzlaff R, Loguercio AD. Impact of adhesive application to wet and dry dentin on long-term resin-dentin bond strengths. Oper Dent. 2007 Jul-Aug;32(4):380-7.

Choi AN, Lee JH, Son S, Jung KH, Kwon Y, Park JK. Effect of dentin wetness on the bond strength of universal adhesives. Materials (Basel). 2017 Oct 25;10(11):1224.

Al Mandouh H, Alzayat I. Sealing of bonded resin composite to gingival wall following application of two hemostatic agents using two adhesive protocols: An in vitro study. Egypt Dent J. 2018 Apr;64:1561-70.

Chaiyabutr Y, Kois JC. The effect of tooth-preparation cleansing protocol on the bond strength of self-adhesive resin cement to dentin contaminated with a hemostatic agent. Oper Dent. 2011 Jan-Feb;36(1):18-26.

Land MF, Couri CC, Johnston WM. Smear layer instability caused by hemostatic agents. The J

Prosthet Dent. 1996 Nov;76(5):477-82.

Saad A, Inoue G, NikaidoT, Abdou AM, Sayed M, Burrow MF, et al. Effect of dentin contamination with two hemostatic agents on bond strength of resin-modified glass ionomer cement with different conditioning. Dent Mater J. 2019 Mar 31;38(2):257-263.

Saati K, Yazdi HK, Valaei N, Bonakdar B, Khodadadzadeh P, Yousefi ES. Effect of aluminium chloride on bond strength of adhesive resins. Biosci. Biotechnol. Res. Commun.. 2018;11(1):83-8.

IssueVolume 18 QRcode
SectionOriginal Article
DOI https://doi.org/10.18502/fid.v18i27.6937
Adhesives Hemostatics; Shear Strength;

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How to Cite
Khamverdi Z, Karimian N, Farhadian M, Gheitouli H. Effect of Contamination with Hemostatic Agent on Shear Bond Strength of Composite to Dentin Using G-Premio and Single Bond Universal Adhesives. Front Dent. 2021;18.