Original Article

Microtensile Bond Strength of Different Bonding Agents to Superficial and Deep Dentin in Etch-and-Rinse and Self-Etch Mode

Bond Strength of Bonding Agent in Different Modes


Objectives: This study aimed to assess the microtensile bond strength (µTBS) of etch-and-rinse (E&R), self-etch (SE), and universal adhesives to superficial and deep dentin.

Materials and Methods: In this in vitro study, 40 sound third molars were randomly divided into two main groups of superficial and deep dentin. Based on our classification, superficial dentin was right beneath the deepest occlusal groove, and deep dentin was 2mm beneath the deepest occlusal groove. Each group was divided into 4 subgroups (n=20) for application of Adper Single Bond 2 (ASB), Clearfil SE Bond (CSE), and Scotchbond Universal (SBU) in E&R and SE modes along with Charisma Smart composite resin on dentin. The specimens were incubated in distilled water at 37°C for 24 hours and their µTBS was then measured. The mode of failure was determined under a stereomicroscope at ×40 magnification. Data were analyzed by one-way ANOVA (alpha=0.05).

Results: The highest µTBS belonged to the superficial dentin/SBU/E&R group. The µTBS was significantly higher in superficial dentin than deep dentin for all adhesives (P=0.005). There was no significant difference in mode of failure among the groups.

Conclusion: Based on the results obtained in the present study, type of bonding agent and application mode affected µTBS. In use of universal adhesive, E&R mode can improve µTBS.

1. Mackenzie L, Banerjee A. Minimally invasive direct restorations: a practical guide. Br Dent J. 2017 Aug 11;223(3):163-171.
2. Tjäderhane L. Dentin bonding: can we make it last? Oper Dent. 2015 Jan-Feb;40(1):4-18.
3. Orellana N, Ramírez R, Roig M, Giner L, Mercade M, Durán F, et al. Comparative study of the microtensile bond strength of three different total etch adhesives with different solvents to wet and dry dentin (in vitro test). Acta Odontol Latinoam. 2009;22(1):47-56.
4. Burrow MF, Takakura H, Nakajima M, Inai N, Tagami J, Takatsu T. The influence of age and depth of dentin on bonding. Dent Mater. 1994 Jul;10(4):241-6.
5. Rosa WL, Piva E, Silva AF. Bond strength of universal adhesives: A systematic review and meta-analysis. J Dent. 2015 Jul;43(7):765-76.
6. Gre C, de Andrada MA, Junior SM. Microtensile bond strength of a universal adhesive to deep dentin. Brazilian Dental Science. 2016 Jul 7;19(2):104-10.
7. Sofan E, Sofan A, Palaia G, Tenore G, Romeo U, Migliau G. Classification review of dental adhesive systems: from the IV generation to the universal type. Ann Stomatol (Roma). 2017 Jul 3;8(1):1-17.
8. Freedman G. Adhesion: past, present, and future. Oral Health. 2019 Jul;109(7):8-14.
9. Kumari RV, Siddaraju K, Nagaraj H, Poluri RK. Evaluation of Shear Bond Strength of Newer Bonding Systems on Superficial and Deep Dentin. J Int Oral Health. 2015 Sep;7(9):31-5.
10. Sirisha K, Rambabu T, Ravishankar Y, Ravikumar P. Validity of bond strength tests: A critical review-Part II. J Conserv Dent. 2014 Sep;17(5):420-6.
11. Pashley EL, Tao L, Matthews WG, Pashley DH. Bond strengths to superficial, intermediate and deep dentin in vivo with four dentin bonding systems. Dent Mater. 1993 Jan;9(1):19-22.
12. Tagami J, Tao L, Pashley DH. Correlation among dentin depth, permeability, and bond strength of adhesive resins. Dent Mater. 1990 Jan;6(1):45-50.
13. Suzuki T, Finger WJ. Dentin adhesives: site of dentin vs. bonding of composite resins. Dent Mater. 1988 Dec;4(6):379-83.
14. Navyasri K, Alla RK, Vineeth G, Suresh Sajjan MC. An overview of dentin bonding agents. Int. J Dent Mater. 2019 Nov 15;1(2):60-7.
15. Jacker-Guhr S, Sander J, Luehrs AK. How "Universal" is Adhesion? Shear Bond Strength of Multi-mode Adhesives to Enamel and Dentin. J Adhes Dent. 2019;21(1):87-95.
16. Yeşilyurt C, Bulucu B. Bond strength of total-etch and self-etch dentin adhesive systems on peripheral and central dentinal tissue: a microtensile bond strength test. J Contemp Dent Pract. 2006 May 1;7(2):26-36.
17. Yaseen SM, Subba Reddy VV. Comparative evaluation of shear bond strength of two self-etching adhesives (sixth and seventh generation) on dentin of primary and permanent teeth: an in vitro study. J Indian Soc Pedod Prev Dent. 2009 Jan-Mar;27(1):33-8.
18. Ramachandran A, Varughese JM. Correlation of shear bond strength with resin tag length and hybrid layer thickness at the resin dentine interface using a Total etch & Self etch bonding agents-An invitro Study. International Journal of Scientific & Engineering Research. 2020;11(5):855- 64.
19. Jang JH, Lee MG, Woo SU, Lee CO, Yi JK, Kim DS. Comparative study of the dentin bond strength of a new universal adhesive. Dent Mater J. 2016;35(4):606-12.
IssueVol 20 (Continuously Published Article-Based) QRcode
SectionOriginal Article
DOI https://doi.org/10.18502/fid.v20i9.12608
Composite Resins Dentin-Bonding Agents Dentin

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Sharifian A, Esmaeili B, Gholinia H, Ezoji F. Microtensile Bond Strength of Different Bonding Agents to Superficial and Deep Dentin in Etch-and-Rinse and Self-Etch Mode. Front Dent. 2023;20.