In Vitro Effects of Chlorhexidine and Isopropyl Alcohol Conditioning Agents on Immediate and Late Bond Strength of Fiber Posts to Dentin
Abstract
Objectives: This study assessed the effect of chlorhexidine (CHX) and isopropyl alcohol (IA) on immediate and late pushout bond strength (PBS) of fiber posts to dentin.
Materials and Methods: In this in vitro study, 54 single-canal premolars were endodontically treated, and randomly assigned to 3 groups (N=18) for root dentin conditioning with distilled water (control), 2% CHX, and 70% IA after post space preparation. Fiber posts were cemented with TheraCem self-adhesive cement, and each group was subdivided into two subgroups (N=9) for PBS measurement immediately after bonding, and after 5000 thermal cycles (5-55°C). The roots were then sectioned, and their PBS was measured. The mode of failure was evaluated under a stereomicroscope at ×40 magnification. Data were analyzed by repeated measures ANOVA and Tukey’s test (alpha=0.05).
Results: The highest PBS was noted in the IA group (21.12 MPa) after 24 hours and the lowest PBS belonged to the control group after thermocycling (7.48 MPa). The immediate and post-thermocycling PBS were significantly lower in the control group than the CHX group (P<0.05). The PBS in both the control and CHX groups was lower than that in the IA group (P<0.001). Regardless of the type of detergent, a significant reduction in PBS was observed after thermocycling (P<0.003). The PBS significantly decreased from the cervical towards the apical region in all groups (P<0.001).
Conclusion: According to the results, application of IA before the self-adhesive cement effectively improved the immediate and late PBS, and was significantly more effective than CHX.
1. Lecardelli EA, Poletto-Neto V, Pappen FG, Pereira-Cenci T, Morgental RD. Effect of chlorhexidine and isopropyl alcohol on immediate and delayed bond strength of glass fiber posts. G Ital Endod. 2019 Oct;33(2):55-61.
2. Bitter K, Kielbassa AM. Post-endodontic restorations with adhesively luted fiber-reinforced composite post systems: a review. Am J Dent. 2007 Dec;20(6):353-60.
3. Reis KR, Spyrides GM, Oliveira JAd, Jnoub AA, Dias KRHC, Bonfantes G. Effect of cement type and water storage time on the push-out bond strength of a glass fiber post. Braz Dent J. 2011 Oct;22(5):359-64.
4. Stanislawczuk R, Reis A, Loguercio AD. A 2-year in vitro evaluation of a chlorhexidine-containing acid on the durability of resin–dentin interfaces. J Dent. 2010 Oct;39(1):40-7.
5. Durski M, Metz M, Crim G, Hass S, Mazur R, Vieira S. Effect of chlorhexidine treatment prior to fiber post cementation on long-term resin cement bond strength. Oper Dent. 2018 Mar/Apr;43(2):E72-E80.
6. Zmener O, Pameijer CH, Serrano SA, Vidueira M, Macchi RL. Significance of moist root canal dentin with the use of methacrylate-based endodontic sealers: an in vitro coronal dye leakage study. J Endod. 2008 Jan;34(1):76-9.
7. Cecchin D, Giacomin M, Farina AP, Brilhante Bhering CL, Ferraz Mesquita M, Randi Ferraz CC. Effect of chlorhexidine and ethanol on push-out bond strength of fiber posts under cyclic loading. J Adhes Dent. 2014 Feb;16(1):87-92.
8. Engel GT, Goodell GG, McClanahan SB. Sealer penetration and apical microleakage in smear-free dentin after a final rinse with either 70% isopropyl alcohol or Peridex. J Endod. 2005 Aug;31(8):620-3.
9. Okuda M, Pereira P, Nakajima M, Tagami J, Pashley DH. Long-term durability of resin dentin interface: Nanoleakage vs microtensile bond strength. Oper Dent. 2002 May-Jun;27(3):289-96.
10. Bitter K, Meyer-Lueckel H, Priehn K, Kanjuparambil J, Neumann K, Kielbassa A. Effects of luting agent and thermocycling on bond strengths to root canal dentine. Int Endod J. 2006 Oct;39(10):809-18.
11. Chen L, Yang J, Wang JR, Suh BI. Physical and biological properties of a newly developed calcium silicate-based self-adhesive cement. Am J Dent. 2018 Apr;31(2):86-90.
12. Kahnamouei MA, Mohammadi N, Navimipour EJ, Shakerifar M. Push-out bond strength of quartz fibre posts to root canal dentin using total-etch and self-adhesive resin cements. Med Oral Patol Oral Cir Bucal. 2012 Mar;17(2):e337-44.
13. Goes Y, Valandro LF, Souza RO, Correia AC, Jacques LB, Mallmann A. Resin push-out bond between fiber post and root dentin: Evaluation of different cementation approaches. Arch Int Surg. 2013 Dec;3(2):112-18.
14. Nemati Anaraki S, Hoorizad Ganjkar M, Sedighi M. Comparing the Effect of Self-adhesive Resin Cements and Self-etching Bonding System on Retentive Strength of DT light Fiber Post: an in-vitro study. J Res Dent Sci. 2014 Autumn;11(3):142-51.
15. Bitter K, Aschendorff L, Neumann K, Blunck U, Sterzenbach G. Do chlorhexidine and ethanol improve bond strength and durability of adhesion of fiber posts inside the root canal? Clin Oral Investig. 2014 Apr;18(3):927-34.
16. Montagner A, Sarkis-Onofre R, Pereira-Cenci T, Cenci M. MMP inhibitors on dentin stability: a systematic review and meta-analysis. J Dent Res. 2014 Aug;93(8):733-43.
17. Van Landuyt K, Yoshida Y, Hirata I, Snauwaert J, De Munck J, Okazaki M, et al. Influence of the chemical structure of functional monomers on their adhesive performance. J Dent Res. 2008 Aug;87(8):757-61.
18. Mahrous A, Radwan MM, Kamel SM. Micro-Shear Bond Strength of Novel MDP Calcium-Fluoride–Releasing Self-Adhesive Resin Cement After Thermocycling. Int J Periodontics Restorative Dent. 2020 May/Jun;40(3):445-55.
19. Rocha RL, Dourado MOA, Dayrell AC, Pinheiro MLP, Câmara JVF, Pierote JJA, et al. Effect of simplified chemical moisture control protocols on the bond durability of fiber posts cemented to root dentin. Braz J Dev. 2020 Aug;6(8):62121-32.
20. Ghazvehi K, Saffarpour A, Habibzadeh S. Effect of pretreatment with matrix metalloproteinase inhibitors on the durability of bond strength of fiber posts to radicular dentin. Clin Exp Dent Res. 2022 Aug;8(4):893-9.
21. Di Hipólito V, Rodrigues FP, Piveta FB, da Cunha Azevedo L, Alonso RCB, Silikas N, et al. Effectiveness of self-adhesive luting cements in bonding to chlorhexidine-treated dentin. Dent Mater. 2012 May;28(5):495-501.
22. Conte T, Andrade GSd, Gadonski AP, Santin DC, Naufel FS. Evaluation of the effect of chlorhexidine and sodium hypochlorite in adhesive cementation of fiber posts-in vitro study. Rev Odontol UNESP. 2019 Oct;48 :e20190020.
23. MuMCU E, Erdemir U, Topcu FT. Comparison of micro push-out bond strengths of two fiber posts luted using simplified adhesive approaches. Dent Mater J. 2010 May; 29(3):286-96.
24. Lindblad RM, Lassila LV, Salo V, Vallittu PK, Tjäderhane L. One year effect of chlorhexidine on bonding of fibre-reinforced composite root canal post to dentine. J Dent. 2012 Sep;40(9):718-22.
25. Brackett WW, Tay FR, Brackett MG, Dib A, Sword RJ, Pashley DH. The effect of chlorhexidine on dentin hybrid layers in vivo. Oper Dent. 2007 Mar-Apr;32(2):107-11.
26. Carvalho MFFd, Yamauti M, Magalhães CSd, Bicalho AA, Soares CJ, Moreira AN. Effect of ethanol-wet bonding on porosity and retention of fiberglass post to root dentin. Braz Oral J. 2020 Mar 27; :34:e020.
27. Farid F, Rostami K, Habibzadeh S, Kharazifard M. Effect of cement type and thickness on push-out bond strength of fiber posts. J Dent Res Dent Clin Dent Prospects. 2018 Fall;12(4):277-82.
28. Simões T, Luque-Martinez Í, Moraes R, Sá A, Loguercio A, Moura S. Longevity of bonding of self-adhesive resin cement to dentin. Oper Dent. 2016 May-Jun;41(3):E64-E72.
29. Moraes A, Sarkis-Onofre R, Moraes R, Cenci M, Soares C, Pereira-Cenci T. Can silanization increase the retention of glass-fiber posts? A systematic review and meta-analysis of in vitro studies. Oper Dent. 2015 Nov-Dec;40(6):567-80.
2. Bitter K, Kielbassa AM. Post-endodontic restorations with adhesively luted fiber-reinforced composite post systems: a review. Am J Dent. 2007 Dec;20(6):353-60.
3. Reis KR, Spyrides GM, Oliveira JAd, Jnoub AA, Dias KRHC, Bonfantes G. Effect of cement type and water storage time on the push-out bond strength of a glass fiber post. Braz Dent J. 2011 Oct;22(5):359-64.
4. Stanislawczuk R, Reis A, Loguercio AD. A 2-year in vitro evaluation of a chlorhexidine-containing acid on the durability of resin–dentin interfaces. J Dent. 2010 Oct;39(1):40-7.
5. Durski M, Metz M, Crim G, Hass S, Mazur R, Vieira S. Effect of chlorhexidine treatment prior to fiber post cementation on long-term resin cement bond strength. Oper Dent. 2018 Mar/Apr;43(2):E72-E80.
6. Zmener O, Pameijer CH, Serrano SA, Vidueira M, Macchi RL. Significance of moist root canal dentin with the use of methacrylate-based endodontic sealers: an in vitro coronal dye leakage study. J Endod. 2008 Jan;34(1):76-9.
7. Cecchin D, Giacomin M, Farina AP, Brilhante Bhering CL, Ferraz Mesquita M, Randi Ferraz CC. Effect of chlorhexidine and ethanol on push-out bond strength of fiber posts under cyclic loading. J Adhes Dent. 2014 Feb;16(1):87-92.
8. Engel GT, Goodell GG, McClanahan SB. Sealer penetration and apical microleakage in smear-free dentin after a final rinse with either 70% isopropyl alcohol or Peridex. J Endod. 2005 Aug;31(8):620-3.
9. Okuda M, Pereira P, Nakajima M, Tagami J, Pashley DH. Long-term durability of resin dentin interface: Nanoleakage vs microtensile bond strength. Oper Dent. 2002 May-Jun;27(3):289-96.
10. Bitter K, Meyer-Lueckel H, Priehn K, Kanjuparambil J, Neumann K, Kielbassa A. Effects of luting agent and thermocycling on bond strengths to root canal dentine. Int Endod J. 2006 Oct;39(10):809-18.
11. Chen L, Yang J, Wang JR, Suh BI. Physical and biological properties of a newly developed calcium silicate-based self-adhesive cement. Am J Dent. 2018 Apr;31(2):86-90.
12. Kahnamouei MA, Mohammadi N, Navimipour EJ, Shakerifar M. Push-out bond strength of quartz fibre posts to root canal dentin using total-etch and self-adhesive resin cements. Med Oral Patol Oral Cir Bucal. 2012 Mar;17(2):e337-44.
13. Goes Y, Valandro LF, Souza RO, Correia AC, Jacques LB, Mallmann A. Resin push-out bond between fiber post and root dentin: Evaluation of different cementation approaches. Arch Int Surg. 2013 Dec;3(2):112-18.
14. Nemati Anaraki S, Hoorizad Ganjkar M, Sedighi M. Comparing the Effect of Self-adhesive Resin Cements and Self-etching Bonding System on Retentive Strength of DT light Fiber Post: an in-vitro study. J Res Dent Sci. 2014 Autumn;11(3):142-51.
15. Bitter K, Aschendorff L, Neumann K, Blunck U, Sterzenbach G. Do chlorhexidine and ethanol improve bond strength and durability of adhesion of fiber posts inside the root canal? Clin Oral Investig. 2014 Apr;18(3):927-34.
16. Montagner A, Sarkis-Onofre R, Pereira-Cenci T, Cenci M. MMP inhibitors on dentin stability: a systematic review and meta-analysis. J Dent Res. 2014 Aug;93(8):733-43.
17. Van Landuyt K, Yoshida Y, Hirata I, Snauwaert J, De Munck J, Okazaki M, et al. Influence of the chemical structure of functional monomers on their adhesive performance. J Dent Res. 2008 Aug;87(8):757-61.
18. Mahrous A, Radwan MM, Kamel SM. Micro-Shear Bond Strength of Novel MDP Calcium-Fluoride–Releasing Self-Adhesive Resin Cement After Thermocycling. Int J Periodontics Restorative Dent. 2020 May/Jun;40(3):445-55.
19. Rocha RL, Dourado MOA, Dayrell AC, Pinheiro MLP, Câmara JVF, Pierote JJA, et al. Effect of simplified chemical moisture control protocols on the bond durability of fiber posts cemented to root dentin. Braz J Dev. 2020 Aug;6(8):62121-32.
20. Ghazvehi K, Saffarpour A, Habibzadeh S. Effect of pretreatment with matrix metalloproteinase inhibitors on the durability of bond strength of fiber posts to radicular dentin. Clin Exp Dent Res. 2022 Aug;8(4):893-9.
21. Di Hipólito V, Rodrigues FP, Piveta FB, da Cunha Azevedo L, Alonso RCB, Silikas N, et al. Effectiveness of self-adhesive luting cements in bonding to chlorhexidine-treated dentin. Dent Mater. 2012 May;28(5):495-501.
22. Conte T, Andrade GSd, Gadonski AP, Santin DC, Naufel FS. Evaluation of the effect of chlorhexidine and sodium hypochlorite in adhesive cementation of fiber posts-in vitro study. Rev Odontol UNESP. 2019 Oct;48 :e20190020.
23. MuMCU E, Erdemir U, Topcu FT. Comparison of micro push-out bond strengths of two fiber posts luted using simplified adhesive approaches. Dent Mater J. 2010 May; 29(3):286-96.
24. Lindblad RM, Lassila LV, Salo V, Vallittu PK, Tjäderhane L. One year effect of chlorhexidine on bonding of fibre-reinforced composite root canal post to dentine. J Dent. 2012 Sep;40(9):718-22.
25. Brackett WW, Tay FR, Brackett MG, Dib A, Sword RJ, Pashley DH. The effect of chlorhexidine on dentin hybrid layers in vivo. Oper Dent. 2007 Mar-Apr;32(2):107-11.
26. Carvalho MFFd, Yamauti M, Magalhães CSd, Bicalho AA, Soares CJ, Moreira AN. Effect of ethanol-wet bonding on porosity and retention of fiberglass post to root dentin. Braz Oral J. 2020 Mar 27; :34:e020.
27. Farid F, Rostami K, Habibzadeh S, Kharazifard M. Effect of cement type and thickness on push-out bond strength of fiber posts. J Dent Res Dent Clin Dent Prospects. 2018 Fall;12(4):277-82.
28. Simões T, Luque-Martinez Í, Moraes R, Sá A, Loguercio A, Moura S. Longevity of bonding of self-adhesive resin cement to dentin. Oper Dent. 2016 May-Jun;41(3):E64-E72.
29. Moraes A, Sarkis-Onofre R, Moraes R, Cenci M, Soares C, Pereira-Cenci T. Can silanization increase the retention of glass-fiber posts? A systematic review and meta-analysis of in vitro studies. Oper Dent. 2015 Nov-Dec;40(6):567-80.
| Issue | Vol 21 (Continuously Published Article-Based) | |
| Section | Original Article | |
| DOI | https://doi.org/10.18502/fid.v21i13.15374 | |
| Keywords | ||
| Chlorhexidine 2-Propanol Dentin Root Canal Therapy | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Emami Arjomand M, Nasoohi N, Shabani M. In Vitro Effects of Chlorhexidine and Isopropyl Alcohol Conditioning Agents on Immediate and Late Bond Strength of Fiber Posts to Dentin. Front Dent. 2024;21.
