Potential Implications of Organoids in Dental Research
organoids and dentistry
,
Abstract
Organoids are among the most significant advancements in the past few years that have revolutionized the medical and dental research. These experimental organs have been used to study the developmental processes, pathophysiology, genetic mutations, and drug effects. The organoid technology is an important adjunct to two-dimensional culture methods and animal models. Stem cells are grown in specific environments to develop the organoids, thus, simulating the complexity of the in vivo environment. Oral organoids also recapitulate the architecture and functions of in vivo organs and tissues in the oral and maxillofacial region. Organoids are a promising tool to manipulate human dental stem cell biology for comprehending tooth-regenerative approaches, infectious diseases, genetic anomalies, oral cancer, and drug discovery.
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4. Schutgens F, Clevers H. Human organoids: Tools for understanding biology and treating diseases. Annu Rev Pathol. 2020 Jan;15:211-34.
5. Rauth S, Karmakar S, Batra SK, Ponnusamy MP. Recent advances in organoid development and applications in disease modeling. BiochimBiophys Acta Rev Cancer. 2021 Apr;1875(2):188527.
6. Clevers H. Modeling development and disease with organoids. Cell. 2016 Jun;165(7):1586-97.
7. Shariati L, Esmaeili Y, Haghjooy Javanmard S, Bidram E, Amini A. Organoid technology: Current standing and future perspectives. Stem Cells. 2021 Dec;39(12):1625-49.
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9. Shankaran A, Prasad K, Chaudhari S, Brand A, Satyamoorthy K. Advances in development and application of human organoids. 3 Biotech. 2021 Jun;11(6):257.
10. Aguilar C, Alves da Silva M, Saraiva M, Neyazi M, Olsson IAS, Bartfeld S. Organoids as host models for infection biology - a review of methods. Exp Mol Med. 2021 Oct;53(10):1471-82.
11. de Poel E, Lefferts JW, Beekman JM. Intestinal organoids for cystic fibrosis research. J Cyst Fibros. 2020 Mar;19 Suppl 1:S60-4.
12. Su X, Yue P, Kong J, Xu X, Zhang Y, Cao W, et al. Human brain organoids as an in vitro model system of viral infectious diseases. Front Immunol. 2022 Jan;12:792316.
13. Kim J, Koo BK, Clevers H. Organoid studies in COVID-19 research. Int J Stem Cells. 2022 Feb;15(1):3-13.
14. Hoarau-Véchot J, Rafii A, Touboul C, Pasquier J. Halfway between 2D and animal models: Are 3D cultures the ideal tool to study cancer-microenvironment interactions? Int J Mol Sci. 2018 Jan;19(1):181.
15. Xu H, Jiao D, Liu A, Wu K. Tumor organoids: applications in cancer modeling and potentials in precision medicine. J Hematol Oncol. 2022 May;15(1):58.
16. Verduin M, Hoeben A, De Ruysscher D, Vooijs M. Patient-derived cancer organoids as predictors of treatment response. Front Oncol. 2021 Mar;11:641980.
17. Kilic Bektas C, Zhang W, Mao Y, Wu X, Kohn J, Yelick PC. Self-assembled hydrogel microparticle-based tooth-germ organoids. Bioengineering (Basel). 2022 May;9(5):215.
18. Rosowski J, Bräunig J, Amler AK, Strietzel FP, Lauster R, Rosowski M. Emulating the early phases of human tooth development in vitro. Sci Rep. 2019 May;9(1):7057.
19. Hemeryck L, Hermans F, Chappell J, Kobayashi H, Lambrechts D, Lambrichts I, et al. Organoids from human tooth showing epithelial stemness phenotype and differentiation potential. Cell Mol Life Sci. 2022 Feb;79(3):153.
20. Alghadeer A, Hanson-Drury S, Ehnes D, Zhao YT, Patni AP, O’Day D, Spurrell CH, Gogate AA, Phal A, Zhang H, Devi A. Human iPSC derived enamel organoid guided by single-cell atlas of human tooth development. Biorxiv. 2022 Aug:2022-08.
21. Xu X, Li Z, Ai X, Tang Y, Yang D, Dou L. Human three-dimensional dental pulp organoid model for toxicity screening of dental materials on dental pulp cells and tissue. Int Endod J. 2022 Jan;55(1):79-88.
22. Jeong SY, Lee S, Choi WH, Jee JH, Kim HR, Yoo J. Fabrication of dentin-pulp-like organoids using dental-pulp stem cells. Cells. 2020 Mar;9(3):642.
23. Chu J, Pieles O, Pfeifer CG, Alt V, Morsczeck C, Docheva D. Dental follicle cell differentiation towards periodontal ligament-like tissue in a self-assembly three-dimensional organoid model. Eur Cell Mater. 2021 Jul;42:20-33.
24. Pringle S, Maimets M, van der Zwaag M, Stokman MA, van Gosliga D, Zwart E, Witjes MJ, de Haan G, van Os R, Coppes RP. Human Salivary Gland Stem Cells Functionally Restore Radiation Damaged Salivary Glands. Stem Cells. 2016 Mar;34(3):640-52.
25. Aihara E, Mahe MM, Schumacher MA, Matthis AL, Feng R, Ren W, et al. Characterization of stem/progenitor cell cycle using murine circumvallate papilla taste bud organoid. Sci Rep. 2015 Nov;5:17185.
26. Hisha H, Tanaka T, Kanno S, Tokuyama Y, Komai Y, Ohe S, et al. Establishment of a novel lingual organoid culture system: generation of organoids having mature keratinized epithelium from adult epithelial stem cells. Sci Rep. 2013 Nov;3:3224.
27. Driehuis E, Kolders S, Spelier S, Lõhmussaar K, Willems SM, Devriese LA, et al. Oral mucosal organoids as a potential platform for personalized cancer therapy. Cancer Discov. 2019 Jul;9(7):852-71.
28. Karakasheva TA, Kijima T, Shimonosono M, Maekawa H, Sahu V, Gabre JT, et al. Generation and characterization of patient-derived head and neck, oral, and esophageal cancer organoids. Curr Protoc Stem Cell Biol. 2020 Jun;53(1):e109.
29. Shoushrah SH, Transfeld JL, Tonk CH, Büchner D, Witzleben S, Sieber MA, et al. Sinking Our teeth in getting dental stem cells to clinics for bone regeneration. Int J Mol Sci. 2021 Jun;22(12):6387.
30. Kim GH, Yang J, Jeon DH, Kim JH, Chae GY, Jang M, et al. Differentiation and establishment of dental epithelial-like stem cells derived from human ESCs and iPSCs. Int J Mol Sci. 2020 Jun;21(12):4384.
31. Hemeryck L, Lambrichts I, Bronckaers A, Vankelecom H. Establishing organoids from human tooth as a powerful tool toward mechanistic research and regenerative therapy. J Vis Exp. 2022 Apr;(182).
32. Li B, Ouchi T, Cao Y, Zhao Z, Men Y. Dental-Derived Mesenchymal Stem Cells: State of the Art. Front Cell Dev Biol. 2021 Jun;9:654559.
33. Chu J, Pieles O, Pfeifer CG, Alt V, Morsczeck C, Docheva D. Dental follicle cell differentiation towards periodontal ligament-like tissue in a self-assembly three-dimensional organoid model. Eur Cell Mater. 2021 Jul;42:20-33.
34. Wang Y, Sun Y. Engineered organoids in oral and maxillofacial regeneration. iScience. 2022 Dec;26(1):105757.
35. Lassche G, van Engen-van Grunsven ACH, van Hooij O, Aalders TW, Am Weijers J, Cocco E, et al. Precision oncology using organoids of a secretory carcinoma of the salivary gland treated with TRK-inhibitors. Oral Oncol. 2023 Feb;137:106297.
36. Blutt SE, Estes MK. Organoid models for infectious disease. Annu Rev Med. 2022 Jan;73:167-82.
37. Chitturi Suryaprakash RT, Kujan O, Shearston K, Farah CS. Three-dimensional cell culture models to investigate oral carcinogenesis: A scoping review. Int J Mol Sci. 2020 Dec;21(24):9520.
38. Mohtasham N, Mohajer Tehran F, Abbaszadeh H. Head and neck cancer organoids as a promising tool for personalized cancer therapy: A literature review. Health Sci Rep. 2022 Apr;5(3):e580.
39. Lo YH, Karlsson K, Kuo CJ. Applications of organoids for cancer biology and precision medicine. Nat Cancer. 2020 Aug;1(8):761-73.
2. Simian M, Bissell MJ. Organoids: A historical perspective of thinking in three dimensions. J Cell Biol. 2017 Jan;216(1):31-40.
3. Corrò C, Novellasdemunt L, Li VSW. A brief history of organoids. Am J Physiol Cell Physiol. 2020 Jul 1;319(1):C151-65.
4. Schutgens F, Clevers H. Human organoids: Tools for understanding biology and treating diseases. Annu Rev Pathol. 2020 Jan;15:211-34.
5. Rauth S, Karmakar S, Batra SK, Ponnusamy MP. Recent advances in organoid development and applications in disease modeling. BiochimBiophys Acta Rev Cancer. 2021 Apr;1875(2):188527.
6. Clevers H. Modeling development and disease with organoids. Cell. 2016 Jun;165(7):1586-97.
7. Shariati L, Esmaeili Y, Haghjooy Javanmard S, Bidram E, Amini A. Organoid technology: Current standing and future perspectives. Stem Cells. 2021 Dec;39(12):1625-49.
8. Lehmann R, Lee CM, Shugart EC, Benedetti M, Charo RA, Gartner Z, et al. Human organoids: a new dimension in cell biology. Mol Biol Cell. 2019 May;30(10):1129-37.
9. Shankaran A, Prasad K, Chaudhari S, Brand A, Satyamoorthy K. Advances in development and application of human organoids. 3 Biotech. 2021 Jun;11(6):257.
10. Aguilar C, Alves da Silva M, Saraiva M, Neyazi M, Olsson IAS, Bartfeld S. Organoids as host models for infection biology - a review of methods. Exp Mol Med. 2021 Oct;53(10):1471-82.
11. de Poel E, Lefferts JW, Beekman JM. Intestinal organoids for cystic fibrosis research. J Cyst Fibros. 2020 Mar;19 Suppl 1:S60-4.
12. Su X, Yue P, Kong J, Xu X, Zhang Y, Cao W, et al. Human brain organoids as an in vitro model system of viral infectious diseases. Front Immunol. 2022 Jan;12:792316.
13. Kim J, Koo BK, Clevers H. Organoid studies in COVID-19 research. Int J Stem Cells. 2022 Feb;15(1):3-13.
14. Hoarau-Véchot J, Rafii A, Touboul C, Pasquier J. Halfway between 2D and animal models: Are 3D cultures the ideal tool to study cancer-microenvironment interactions? Int J Mol Sci. 2018 Jan;19(1):181.
15. Xu H, Jiao D, Liu A, Wu K. Tumor organoids: applications in cancer modeling and potentials in precision medicine. J Hematol Oncol. 2022 May;15(1):58.
16. Verduin M, Hoeben A, De Ruysscher D, Vooijs M. Patient-derived cancer organoids as predictors of treatment response. Front Oncol. 2021 Mar;11:641980.
17. Kilic Bektas C, Zhang W, Mao Y, Wu X, Kohn J, Yelick PC. Self-assembled hydrogel microparticle-based tooth-germ organoids. Bioengineering (Basel). 2022 May;9(5):215.
18. Rosowski J, Bräunig J, Amler AK, Strietzel FP, Lauster R, Rosowski M. Emulating the early phases of human tooth development in vitro. Sci Rep. 2019 May;9(1):7057.
19. Hemeryck L, Hermans F, Chappell J, Kobayashi H, Lambrechts D, Lambrichts I, et al. Organoids from human tooth showing epithelial stemness phenotype and differentiation potential. Cell Mol Life Sci. 2022 Feb;79(3):153.
20. Alghadeer A, Hanson-Drury S, Ehnes D, Zhao YT, Patni AP, O’Day D, Spurrell CH, Gogate AA, Phal A, Zhang H, Devi A. Human iPSC derived enamel organoid guided by single-cell atlas of human tooth development. Biorxiv. 2022 Aug:2022-08.
21. Xu X, Li Z, Ai X, Tang Y, Yang D, Dou L. Human three-dimensional dental pulp organoid model for toxicity screening of dental materials on dental pulp cells and tissue. Int Endod J. 2022 Jan;55(1):79-88.
22. Jeong SY, Lee S, Choi WH, Jee JH, Kim HR, Yoo J. Fabrication of dentin-pulp-like organoids using dental-pulp stem cells. Cells. 2020 Mar;9(3):642.
23. Chu J, Pieles O, Pfeifer CG, Alt V, Morsczeck C, Docheva D. Dental follicle cell differentiation towards periodontal ligament-like tissue in a self-assembly three-dimensional organoid model. Eur Cell Mater. 2021 Jul;42:20-33.
24. Pringle S, Maimets M, van der Zwaag M, Stokman MA, van Gosliga D, Zwart E, Witjes MJ, de Haan G, van Os R, Coppes RP. Human Salivary Gland Stem Cells Functionally Restore Radiation Damaged Salivary Glands. Stem Cells. 2016 Mar;34(3):640-52.
25. Aihara E, Mahe MM, Schumacher MA, Matthis AL, Feng R, Ren W, et al. Characterization of stem/progenitor cell cycle using murine circumvallate papilla taste bud organoid. Sci Rep. 2015 Nov;5:17185.
26. Hisha H, Tanaka T, Kanno S, Tokuyama Y, Komai Y, Ohe S, et al. Establishment of a novel lingual organoid culture system: generation of organoids having mature keratinized epithelium from adult epithelial stem cells. Sci Rep. 2013 Nov;3:3224.
27. Driehuis E, Kolders S, Spelier S, Lõhmussaar K, Willems SM, Devriese LA, et al. Oral mucosal organoids as a potential platform for personalized cancer therapy. Cancer Discov. 2019 Jul;9(7):852-71.
28. Karakasheva TA, Kijima T, Shimonosono M, Maekawa H, Sahu V, Gabre JT, et al. Generation and characterization of patient-derived head and neck, oral, and esophageal cancer organoids. Curr Protoc Stem Cell Biol. 2020 Jun;53(1):e109.
29. Shoushrah SH, Transfeld JL, Tonk CH, Büchner D, Witzleben S, Sieber MA, et al. Sinking Our teeth in getting dental stem cells to clinics for bone regeneration. Int J Mol Sci. 2021 Jun;22(12):6387.
30. Kim GH, Yang J, Jeon DH, Kim JH, Chae GY, Jang M, et al. Differentiation and establishment of dental epithelial-like stem cells derived from human ESCs and iPSCs. Int J Mol Sci. 2020 Jun;21(12):4384.
31. Hemeryck L, Lambrichts I, Bronckaers A, Vankelecom H. Establishing organoids from human tooth as a powerful tool toward mechanistic research and regenerative therapy. J Vis Exp. 2022 Apr;(182).
32. Li B, Ouchi T, Cao Y, Zhao Z, Men Y. Dental-Derived Mesenchymal Stem Cells: State of the Art. Front Cell Dev Biol. 2021 Jun;9:654559.
33. Chu J, Pieles O, Pfeifer CG, Alt V, Morsczeck C, Docheva D. Dental follicle cell differentiation towards periodontal ligament-like tissue in a self-assembly three-dimensional organoid model. Eur Cell Mater. 2021 Jul;42:20-33.
34. Wang Y, Sun Y. Engineered organoids in oral and maxillofacial regeneration. iScience. 2022 Dec;26(1):105757.
35. Lassche G, van Engen-van Grunsven ACH, van Hooij O, Aalders TW, Am Weijers J, Cocco E, et al. Precision oncology using organoids of a secretory carcinoma of the salivary gland treated with TRK-inhibitors. Oral Oncol. 2023 Feb;137:106297.
36. Blutt SE, Estes MK. Organoid models for infectious disease. Annu Rev Med. 2022 Jan;73:167-82.
37. Chitturi Suryaprakash RT, Kujan O, Shearston K, Farah CS. Three-dimensional cell culture models to investigate oral carcinogenesis: A scoping review. Int J Mol Sci. 2020 Dec;21(24):9520.
38. Mohtasham N, Mohajer Tehran F, Abbaszadeh H. Head and neck cancer organoids as a promising tool for personalized cancer therapy: A literature review. Health Sci Rep. 2022 Apr;5(3):e580.
39. Lo YH, Karlsson K, Kuo CJ. Applications of organoids for cancer biology and precision medicine. Nat Cancer. 2020 Aug;1(8):761-73.
| Issue | Vol 22 (Continuously Published Article-Based) | |
| Section | Review Article | |
| Keywords | ||
| Organoids Stem Cells Regenerative Medicine Molecular Targeted Therapy Tissue Engineering | ||
| Rights and permissions | |
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Sharm D, Koshy G, George MS, Sharma V. Potential Implications of Organoids in Dental Research. Front Dent. 2025;22.
