Antimicrobial Properties of Acrylic Resin Incorporated with Propolis Nanoparticles
-
Sepideh Arab
,
Abbas Bahador
,
Ahmad Sodagar
,
Maryam Pourhajibagher
,
Azam Akhavan
,
Aseel Niema Hafith
,
Tahereh Pornamazeh
Abstract
Objectives: One of the main problems with polymethyl methacrylate (PMMA) used for the fabrication of oral removable appliances is plaque accumulation due to surface porosities. Incorporation of antimicrobial agents in this material might help tackle this problem. The aim of this study was to evaluate the antimicrobial activity of PMMA acrylic resin incorporated with propolis nanoparticles (PNPs).
Materials and Methods: Antimicrobial properties of acrylic resin incorporated with PNPs were assessed against Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis), Lactobacillus acidophilus (L. acidophilus) and Candida albicans (C. albicans). Acrylic discs were fabricated in four groups: A control group without PNPs and three experimental groups containing 0.5%, 1% and 2% concentrations of PNPs. Disc agar diffusion (DAD) test was performed to determine the antimicrobial effects of PNPs by measuring the microbial growth inhibition zones on Muller-Hinton agar plates. The eluted components test evaluated the viable counts of microorganisms in liquid medium after 24 and 72h. Finally, biofilm inhibition test assessed the efficacy of PNPs for inhibition of biofilm formation. P<0.05 was considered significant.
Results: The acrylic discs failed to produce microbial inhibition zones in the DAD test. Discs containing 1% and 2% nanoparticles showed anti-biofilm effects on all four microbial species. The colony counts of all microorganisms significantly decreased following exposure to liquids containing nanoparticles after 24 and 72h in eluted component test.
Conclusion: PMMA acrylic discs incorporated with PNPs presented some antimicrobial properties against S. mutans, S. sanguinis, L. acidophilus, and C. albicans
1. Siqueira Gonçalves T, Minghelli Schmitt V, Thomas M, Lopes de Souza MA, Macedo de Menezes L. Cytotoxicity of two autopolymerized acrylic resins used in orthodontics. Angle Orthod. 2008 Sep;78(5):926-30.
2. Lessa FCR, Enoki C, Ito IY, Faria G, Matsumoto MAN, Nelson-Filho P. In-vivo evaluation of the bacterial contamination and disinfection of acrylic baseplates of removable orthodontic appliances Am J Orthod Dentofacial Orthop. 2007 Jun;131(6):705. e11-7.
3. Machorowska-Pieniążek A, Morawiec T, Mertas A, Tanasiewicz M, Dziedzic A, Król W. Influence of propolis on hygiene, gingival condition, and oral microflora in patients with cleft lip and palate treated with fixed orthodontic appliances Evid.-Based Complementary Altern. Med. 2013 Jan;2013: 183915
4. Sodagar A, Khalil S, Kassaee MZ, Shahroudi AS, Pourakbari B, Bahador A. Antimicrobial properties of poly (methyl methacrylate) acrylic resins incorporated with silicon dioxide and titanium dioxide nanoparticles on cariogenic bacteria J. Orthod. Sci. 2016 Jan;5(1):7-13.
5. Chang CS, Al-Awadi S, Ready D, Noar J. An assessment of the effectiveness of mechanical and chemical cleaning of Essix orthodontic retainer. J. Orthod. 2014 Jun;41(2):110-7.
6. Vento-Zahra E, De Wever B, Decelis S, Mallia K, Camilleri S. Randomized, double-blind, placebo-controlled trail to test the efficacy of NitrAdine tablets in maxillary removable orthodontic appliance patients. Quintessence Int. 2011 Jan;42(1):37-43.
7. Fathi H, Martiny H, Jost-Brinkmann PG. Efficacy of cleaning tablets for removable orthodontic appliances J Orofac Orthop.. 2015 Mar;76(2):143-51.
8. Liu SY, Tonggu L, Niu LN, Gong SQ, Fan B, Wang L, et al. Antimicrobial activity of a quaternary ammonium methacryloxy silicate-containing acrylic resin: a randomised clinical trial. Sci. Rep.2016 Feb; 6(1):1-4.
9. Cohen WJ, Wiltshire WA, Dawes C, Lavelle CL. Long-term in vitro fluoride release and rerelease from orthodontic bonding materials containing fluoride. Am J Orthod Dentofacial Orthop. 2003 Nov;124(5):571-6.
10. Jedrychowski JR, Caputo AA, Kerper S. Antibacterial and mechanical properties of restorative materials combined with chlorhexidines. J Oral Rehabil. 1983 Sep;10(5):373-81.
11. Redlich M, Katz A, Rapoport L, Wagner H, Feldman Y, Tenne R. Improved orthodontic stainless steel wires coated with inorganic fullerene-like nanoparticles of WS2 impregnated in electroless nickel–phosphorous film. Dent Mater. 2008 Dec;24(12):1640-6.
12. Miura KK, Ito IY, Enoki C, Elias AM, Matsumoto MAN. Anticariogenic effect of fluoride-releasing elastomers in orthodontic patients. Braz Oral Res. 2007;21(3):228-33.
13. Gunes IS, Jana SC. Shape memory polymers and their nanocomposites: a review of science and technology of new multifunctional materials. J. Nanosci. Nanotechnol. 2008 Apr;8(4):1616-37.
14. Stuart MAC, Huck WT, Genzer J, Müller M, Ober C, Stamm M, et al. Emerging applications of stimuli-responsive polymer materials. Nat. Mater.2010 Feb;9(2):101-13.
15. Allaker R. The use of nanoparticles to control oral biofilm formation. J. Dent. Res. 2010 Nov;89(11):1175-86.
16. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002 Jul;10(3).
17. Moreno MN, Isla M, Cudmani N, Vattuone M, Sampietro A. Screening of antibacterial activity of Amaicha del Valle (Tucumán, Argentina) propolis. J Ethnopharmacol. 1999 Dec;68(1-3):97-102.
18. Viuda‐Martos M, Ruiz‐Navajas Y, Fernández‐López J, Pérez‐Álvarez J. Functional properties of honey, propolis, and royal jelly J. Food Sci. 2008 Nov;73(9):R117-24.
19. Dziedzic A, Kubina R, Wojtyczka RD, Kabała-Dzik A, Tanasiewicz M, Morawiec T. The antibacterial effect of ethanol extract of polish propolis on mutans streptococci and lactobacilli isolated from saliva. Evid. Based Complement. Alternat. Med. 2013 Oct;2013: 681891
20. Ikeno K, Ikeno T, Miyazawa C. Effects of propolis on dental caries in rats. Caries Res.1991;25(5):347-51.
21. Koo H, Rosalen P, Cury J, Park Y, Ikegaki M, Sattler A. Effect of Apis mellifera propolis from two Brazilian regions on caries development in desalivated rats. Caries Res. 1999;33(5):393-400.
22. Bretz WA, Chiego D, Marcucci M, Cunha I, Custodio A, Schneider L. Preliminary report on the effects of propolis on wound healing in the dental pulp. Z. Naturforsch. C. 1998 Dec;53(11-12):1045-8.
23. Ozan F, Sümer Z, Polat Z, Er K, Ozan U, Deger O. Effect of mouthrinse containing propolis on oral microorganisms and human gingival fibroblasts. Eur. J. Dent. 2007 Oct;1(4):195-201.
24. Miles AA, Misra S, Irwin J. The estimation of the bactericidal power of the blood. Epidemiol. Infect. 1938 Nov;38(6):732-49.
25. Bahador A, Pourakbari B, Ghorbanzadeh R, Moghadam SO, Sodagar A. Anti-bacterial effects of polymethylmethacrylate with in situ generated silver nanoparticles on primary colonizers of human dental plaque and cariogenic bacteria. Annu. Res. Rev. Biol. 2014 Feb:1587-601.
26. Nam KY. Characterization and bacterial anti-adherent effect on modified PMMA denture acrylic resin containing platinum nanoparticles. J Adv Prosthodont. 2014 Jun;6(3):207-14.
27. Gad MM, Abualsaud R. Behavior of PMMA denture base materials containing titanium dioxide nanoparticles: A literature review. Int. J. Biomater. 2019 Jan;2019: 6190610.
28. Farhadian N, Mashoof RU, Khanizadeh S, Ghaderi E, Farhadian M, Miresmaeili A. Streptococcus mutans counts in patients wearing removable retainers with silver nanoparticles vs those wearing conventional retainers: A randomized clinical trial. Am J Orthod Dentofacial Orthop. 2016 Feb;149(2):155-60.
29. Krol W, Scheller S, Shani J, Pietsz G, Czuba Z. Synergistic effect of ethanolic extract of propolis and antibiotics on the growth of staphylococcus aureus. Arzneimittel-Forschung. 1993 May;43(5):607-9.
30. Kujumgiev A, Bankova V, Ignatova A, Popov S. Antibacterial activity of propolis, some of its components and their analogs. Pharmazie. 1993;48(10):785-6.
31. Ercan N, Erdemir EO, Ozkan SY, Hendek MK. The comparative effect of propolis in two different vehicles; mouthwash and chewing-gum on plaque accumulation and gingival inflammation. Eur J Dent. 2015 Apr;9(2):272-6.
32. Anauate-Netto C, Anido-Anido A, Leegoy HR, Matsumoto R, Alonso RCB, Marcucci MC, et al. Randomized, double-blind, placebo-controlled clinical trial on the effects of propolis and chlorhexidine mouthrinses on gingivitis. Braz. Dent. Sci.. 2014 Jan;17(1):11-5.
33. Sodagar A, Akhavan A, Arab S, Bahador A, Pourhajibagher M, Soudi A. Evaluation of the effect of propolis nanoparticles on antimicrobial properties and shear bond strength of orthodontic composite bonded to bovine enamel. Front Dent. 2019 Mar;16(2):96-104.
34. Ghahremanloo A, Movahedzadeh M. The Effect of Silver Nano Particles on Candida albicans and Streptococcus mutans in Denture Acrylic Resins. J Dent Mater Tech. 2016 Mar;5(1):23-30.
35. Sodagar A, Bahador A, Pourhajibagher M, Ahmadi B, Baghaeian P. Effect of addition of curcumin nanoparticles on antimicrobial property and shear bond strength of orthodontic composite to bovine enamel. J Dent (Tehran). 2016 Sep;13(5):373-82.
36. Aydin Sevinç B, Hanley L. Antibacterial activity of dental composites containing zinc oxide nanoparticles. J Biomed Mater Res B. 2010 Jul;94(1):22-31.
37. Mirhashemi A, Bahador A, Kassaee M, Daryakenari G, Ahmad-Akhoundi M, Sodagar A. Antimicrobial effect of nano-zinc oxide and nano-chitosan particles in dental composite used in orthodontics. J Med Bacteriol. 2013;2(3-4):1-10.
38. Choi O, Yu CP, Fernández GE, Hu Z. Interactions of nanosilver with Escherichia coli cells in planktonic and biofilm cultures. Water Res. 2010 Dec;44(20):6095-103.
39. Becker MR, Paster BJ, Leys EJ, Moeschberger ML, Kenyon SG, Galvin JL, et al. Molecular analysis of bacterial species associated with childhood caries. J. Clin. Microbiol. 2002 Mar;40(3):1001-9.
40. Kreth J, Merritt J, Shi W, Qi F. Competition and coexistence between Streptococcus mutans and Streptococcus sanguinis in the dental biofilm. J. Bacteriol. 2005 Nov;187(21):7193-203.
2. Lessa FCR, Enoki C, Ito IY, Faria G, Matsumoto MAN, Nelson-Filho P. In-vivo evaluation of the bacterial contamination and disinfection of acrylic baseplates of removable orthodontic appliances Am J Orthod Dentofacial Orthop. 2007 Jun;131(6):705. e11-7.
3. Machorowska-Pieniążek A, Morawiec T, Mertas A, Tanasiewicz M, Dziedzic A, Król W. Influence of propolis on hygiene, gingival condition, and oral microflora in patients with cleft lip and palate treated with fixed orthodontic appliances Evid.-Based Complementary Altern. Med. 2013 Jan;2013: 183915
4. Sodagar A, Khalil S, Kassaee MZ, Shahroudi AS, Pourakbari B, Bahador A. Antimicrobial properties of poly (methyl methacrylate) acrylic resins incorporated with silicon dioxide and titanium dioxide nanoparticles on cariogenic bacteria J. Orthod. Sci. 2016 Jan;5(1):7-13.
5. Chang CS, Al-Awadi S, Ready D, Noar J. An assessment of the effectiveness of mechanical and chemical cleaning of Essix orthodontic retainer. J. Orthod. 2014 Jun;41(2):110-7.
6. Vento-Zahra E, De Wever B, Decelis S, Mallia K, Camilleri S. Randomized, double-blind, placebo-controlled trail to test the efficacy of NitrAdine tablets in maxillary removable orthodontic appliance patients. Quintessence Int. 2011 Jan;42(1):37-43.
7. Fathi H, Martiny H, Jost-Brinkmann PG. Efficacy of cleaning tablets for removable orthodontic appliances J Orofac Orthop.. 2015 Mar;76(2):143-51.
8. Liu SY, Tonggu L, Niu LN, Gong SQ, Fan B, Wang L, et al. Antimicrobial activity of a quaternary ammonium methacryloxy silicate-containing acrylic resin: a randomised clinical trial. Sci. Rep.2016 Feb; 6(1):1-4.
9. Cohen WJ, Wiltshire WA, Dawes C, Lavelle CL. Long-term in vitro fluoride release and rerelease from orthodontic bonding materials containing fluoride. Am J Orthod Dentofacial Orthop. 2003 Nov;124(5):571-6.
10. Jedrychowski JR, Caputo AA, Kerper S. Antibacterial and mechanical properties of restorative materials combined with chlorhexidines. J Oral Rehabil. 1983 Sep;10(5):373-81.
11. Redlich M, Katz A, Rapoport L, Wagner H, Feldman Y, Tenne R. Improved orthodontic stainless steel wires coated with inorganic fullerene-like nanoparticles of WS2 impregnated in electroless nickel–phosphorous film. Dent Mater. 2008 Dec;24(12):1640-6.
12. Miura KK, Ito IY, Enoki C, Elias AM, Matsumoto MAN. Anticariogenic effect of fluoride-releasing elastomers in orthodontic patients. Braz Oral Res. 2007;21(3):228-33.
13. Gunes IS, Jana SC. Shape memory polymers and their nanocomposites: a review of science and technology of new multifunctional materials. J. Nanosci. Nanotechnol. 2008 Apr;8(4):1616-37.
14. Stuart MAC, Huck WT, Genzer J, Müller M, Ober C, Stamm M, et al. Emerging applications of stimuli-responsive polymer materials. Nat. Mater.2010 Feb;9(2):101-13.
15. Allaker R. The use of nanoparticles to control oral biofilm formation. J. Dent. Res. 2010 Nov;89(11):1175-86.
16. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002 Jul;10(3).
17. Moreno MN, Isla M, Cudmani N, Vattuone M, Sampietro A. Screening of antibacterial activity of Amaicha del Valle (Tucumán, Argentina) propolis. J Ethnopharmacol. 1999 Dec;68(1-3):97-102.
18. Viuda‐Martos M, Ruiz‐Navajas Y, Fernández‐López J, Pérez‐Álvarez J. Functional properties of honey, propolis, and royal jelly J. Food Sci. 2008 Nov;73(9):R117-24.
19. Dziedzic A, Kubina R, Wojtyczka RD, Kabała-Dzik A, Tanasiewicz M, Morawiec T. The antibacterial effect of ethanol extract of polish propolis on mutans streptococci and lactobacilli isolated from saliva. Evid. Based Complement. Alternat. Med. 2013 Oct;2013: 681891
20. Ikeno K, Ikeno T, Miyazawa C. Effects of propolis on dental caries in rats. Caries Res.1991;25(5):347-51.
21. Koo H, Rosalen P, Cury J, Park Y, Ikegaki M, Sattler A. Effect of Apis mellifera propolis from two Brazilian regions on caries development in desalivated rats. Caries Res. 1999;33(5):393-400.
22. Bretz WA, Chiego D, Marcucci M, Cunha I, Custodio A, Schneider L. Preliminary report on the effects of propolis on wound healing in the dental pulp. Z. Naturforsch. C. 1998 Dec;53(11-12):1045-8.
23. Ozan F, Sümer Z, Polat Z, Er K, Ozan U, Deger O. Effect of mouthrinse containing propolis on oral microorganisms and human gingival fibroblasts. Eur. J. Dent. 2007 Oct;1(4):195-201.
24. Miles AA, Misra S, Irwin J. The estimation of the bactericidal power of the blood. Epidemiol. Infect. 1938 Nov;38(6):732-49.
25. Bahador A, Pourakbari B, Ghorbanzadeh R, Moghadam SO, Sodagar A. Anti-bacterial effects of polymethylmethacrylate with in situ generated silver nanoparticles on primary colonizers of human dental plaque and cariogenic bacteria. Annu. Res. Rev. Biol. 2014 Feb:1587-601.
26. Nam KY. Characterization and bacterial anti-adherent effect on modified PMMA denture acrylic resin containing platinum nanoparticles. J Adv Prosthodont. 2014 Jun;6(3):207-14.
27. Gad MM, Abualsaud R. Behavior of PMMA denture base materials containing titanium dioxide nanoparticles: A literature review. Int. J. Biomater. 2019 Jan;2019: 6190610.
28. Farhadian N, Mashoof RU, Khanizadeh S, Ghaderi E, Farhadian M, Miresmaeili A. Streptococcus mutans counts in patients wearing removable retainers with silver nanoparticles vs those wearing conventional retainers: A randomized clinical trial. Am J Orthod Dentofacial Orthop. 2016 Feb;149(2):155-60.
29. Krol W, Scheller S, Shani J, Pietsz G, Czuba Z. Synergistic effect of ethanolic extract of propolis and antibiotics on the growth of staphylococcus aureus. Arzneimittel-Forschung. 1993 May;43(5):607-9.
30. Kujumgiev A, Bankova V, Ignatova A, Popov S. Antibacterial activity of propolis, some of its components and their analogs. Pharmazie. 1993;48(10):785-6.
31. Ercan N, Erdemir EO, Ozkan SY, Hendek MK. The comparative effect of propolis in two different vehicles; mouthwash and chewing-gum on plaque accumulation and gingival inflammation. Eur J Dent. 2015 Apr;9(2):272-6.
32. Anauate-Netto C, Anido-Anido A, Leegoy HR, Matsumoto R, Alonso RCB, Marcucci MC, et al. Randomized, double-blind, placebo-controlled clinical trial on the effects of propolis and chlorhexidine mouthrinses on gingivitis. Braz. Dent. Sci.. 2014 Jan;17(1):11-5.
33. Sodagar A, Akhavan A, Arab S, Bahador A, Pourhajibagher M, Soudi A. Evaluation of the effect of propolis nanoparticles on antimicrobial properties and shear bond strength of orthodontic composite bonded to bovine enamel. Front Dent. 2019 Mar;16(2):96-104.
34. Ghahremanloo A, Movahedzadeh M. The Effect of Silver Nano Particles on Candida albicans and Streptococcus mutans in Denture Acrylic Resins. J Dent Mater Tech. 2016 Mar;5(1):23-30.
35. Sodagar A, Bahador A, Pourhajibagher M, Ahmadi B, Baghaeian P. Effect of addition of curcumin nanoparticles on antimicrobial property and shear bond strength of orthodontic composite to bovine enamel. J Dent (Tehran). 2016 Sep;13(5):373-82.
36. Aydin Sevinç B, Hanley L. Antibacterial activity of dental composites containing zinc oxide nanoparticles. J Biomed Mater Res B. 2010 Jul;94(1):22-31.
37. Mirhashemi A, Bahador A, Kassaee M, Daryakenari G, Ahmad-Akhoundi M, Sodagar A. Antimicrobial effect of nano-zinc oxide and nano-chitosan particles in dental composite used in orthodontics. J Med Bacteriol. 2013;2(3-4):1-10.
38. Choi O, Yu CP, Fernández GE, Hu Z. Interactions of nanosilver with Escherichia coli cells in planktonic and biofilm cultures. Water Res. 2010 Dec;44(20):6095-103.
39. Becker MR, Paster BJ, Leys EJ, Moeschberger ML, Kenyon SG, Galvin JL, et al. Molecular analysis of bacterial species associated with childhood caries. J. Clin. Microbiol. 2002 Mar;40(3):1001-9.
40. Kreth J, Merritt J, Shi W, Qi F. Competition and coexistence between Streptococcus mutans and Streptococcus sanguinis in the dental biofilm. J. Bacteriol. 2005 Nov;187(21):7193-203.
| Issue | Vol 18 (Continuously Published Article-Based) | |
| Section | Original Article | |
| DOI | https://doi.org/10.18502/fid.v18i29.6939 | |
| Keywords | ||
| Anti-Infective Agents; Nanoparticles; Orthodontics; Polymethyl Methacrylate; Propolis | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Arab S, Bahador A, Sodagar A, Pourhajibagher M, Akhavan A, Hafith AN, Pornamazeh T. Antimicrobial Properties of Acrylic Resin Incorporated with Propolis Nanoparticles. Front Dent. 2021;18.
