Original Article

Comparison of Antibacterial Activities of ProRoot MTA, OrthoMTA, and RetroMTA Against Three Anaerobic Endodontic Bacteria


Objectives: The aim of this study was to assess the antibacterial activities of OrthoMTA, RetroMTA, and ProRoot MTA against Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), and Prevotella intermedia (Pi).
Materials and Methods: Each material was mixed on a glass slab using a spatula and was placed in columns containing the filter membrane of the modified membrane-enclosed immersion test (MEIT) system. The materials were sterilized after setting. The columns containing the sterilized test materials were placed in microcentrifuge tubes containing 500 µl of bacterial suspension. The systems were then incubated at 37°C under anaerobic conditions. After 72 hours, the bacterial growth and concentration (colony-forming unit (CFU)/ml) were assessed. The results were analyzed using one-way analysis of variance (ANOVA) and Tukey's post-hoc test in SPSS 22 software. In all analyses, the differences were considered significant at P<0.05.
Results: OrthoMTA had the highest antibacterial activity against Pi. The mean number of CFU/ml of Fn in the presence of ProRoot MTA and RetroMTA was significantly lower than that in positive controls. There were significant differences between the antibacterial activities of ProRoot MTA and OrthoMTA against Pg compared to positive controls.
Conclusions: ProRoot MTA, OrthoMTA, and RetroMTA had similar antibacterial activities against the three evaluated anaerobic endodontic bacteria, except RetroMTA against Pg.


1- Baumgartner JC, Falkler WA Jr. Bacteria in the apical 5 mm of infected root canals. J Endod. 1991 Aug;17(8):380-3.
2- Siqueira JF Jr, Rôças IN, Souto R, de Uzeda M, Colombo AP. Checkerboard DNA-DNA hybridization analysis of endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2000 Jun;89(6):744-8.
3- Fouad AF, Zerella J, Barry J, Spångberg LS. Molecular detection of Enterococcus species in root canals of therapy-resistant endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005 Jan;99(1):112-8.
4- Kim RJ, Kim MO, Lee KS, Lee DY, Shin JH. An in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (MTA) against five oral bacteria. Arch Oral Biol. 2015 Oct;60(10):1497-502.
5- Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review--part III: Clinical applications, drawbacks, and mechanism of action. J Endod. 2010 Mar;36(3):400-13.
6- Belobrov I, Parashos P. Treatment of tooth discoloration after the use of white mineral trioxide aggregate. J Endod. 2011 Jul;37(7):1017-20.
7- Santos AD, Moraes JC, Araújo EB, Yukimitu K, Valério Filho WV. Physico‐chemical properties of MTA and a novel experimental cement. Int Endod J. 2005 Jul;38(7):443-7.
8- BioMTA. Biofilling starter kit. Available at: http://www.biomta.com/shop/eng/product_1.php/ Accessed January 10, 2017.
9- Stowe TJ, Sedgley CM, Stowe B, Fenno JC. The effects of chlorhexidine gluconate (0.12%) on the antimicrobial properties of tooth-colored ProRoot mineral trioxide aggregate. J Endod. 2004 Jun;30(6):429-31.
10- Holt DM, Watts JD, Beeson TJ, Kirkpatrick TC, Rutledge RE. The anti-microbial effect against enterococcus faecalis and the compressive strength of two types of mineral trioxide aggregate mixed with sterile water or 2% chlorhexidine liquid. J Endod. 2007 Jul;33(7):844-7.
11- Zhang H, Pappen FG, Haapasalo M. Dentin enhances the antibacterial effect of mineral trioxide aggregate and bioaggregate. J Endod. 2009 Feb;35(2):221-4.
12- Odabaş ME, Cinar C, Akça G, Araz I, Ulusu T, Yücel H. Short‐term antimicrobial properties of mineral trioxide aggregate with incorporated silver‐zeolite. Dent Traumatol. 2011 Jun;27(3):189-94.
13- Pereira CV, Stipp RN, Fonseca DC, Pereira LJ, Höfling JF. Detection and clonal analysis of anaerobic bacteria associated to endodontic-periodontal lesions. J Periodontol. 2011 Dec;82(12):1767-75.
14- Bahador A, Pourakbari B, Bolhari B, Hashemi FB. In vitro evaluation of the antimicrobial activity of nanosilver-mineral trioxide aggregate against frequent anaerobic oral pathogens by a membrane-enclosed immersion test. Biomed J. 2015 Jan-Feb;38(1):77-83.
15- BioMTA. Physicochemical Analysis. Available at: http://www.biomta.com/shop/eng/technology_1.php/ Accessed January 15, 2017.
16- Kang SH, Shin YS, Lee HS, Kim SO, Shin Y, Jung IY, et al. Color changes of teeth after treatment with various mineral trioxide aggregate-based materials: an ex vivo study. J Endod. 2015 May;41(5):737-41.
17- Kang EH, Yoo JS, Kim BH, Choi SW, Hong SH. Synthesis and hydration behavior of calcium zirconium aluminate (Ca7ZrAl6O18) cement. Cem Concr Res. 2014 Feb;56:106-11.
18- Lee BN, Son HJ, Noh HJ, Koh JT, Chang HS, Hwang IN, et al. Cytotoxicity of newly developed ortho MTA root-end filling materials. J Endod. 2012 Dec;38(12):1627-30.
19- Donyavi Z, Heidari N, Khoshbin E, Shahriari S, Farhadian M, Yousefi Mashouf R, et al. Antibacterial activity of mineral trioxide aggregate, new endodontic cement, Retro MTA and Ortho MTA against common endodontic pathogens. IAJPS. 2017;4(12):4720-8.
20- Kouchak Dezfouli N, Asnaashari E, Khalilak Z. Comparison of the biocompatibility of Pro Root MTA, Retro MTA and MTA Plus using an MTT assay study. EC Dent Sci. 2017 Jun;11(3):83-7.
21- Sundqvist G, Johansson E, Sjögren U. Prevalence of black-pigmented bacteroides species in root canal infections. J Endod. 1989 Jan;15(1):13-9.
22- Yoshida M, Fukushima H, Yamamoto K, Ogawa K, Toda T, Sagawa H. Correlation between clinical symptoms and microorganisms isolated from root canals of teeth with periapical pathosis. J Endod. 1987 Jan;13(1):24-8.
23- Joshi VM, Vandana KL. The detection of eight putative periodontal pathogens in adult and rapidly progressive periodontitis patients: an institutional study. Indian J Dent Res. 2007 Jan-Mar;18(1):6-10.
24- Komabayashi T, Spångberg LS. Particle size and shape analysis of MTA finer fractions using Portland cement. J Endod. 2008 Jun;34(6):709-11.
25- Kim M, Yang W, Kim H, Ko H. Comparison of the biological properties of ProRoot MTA, OrthoMTA, and Endocem MTA cements. J Endod. 2014 Oct;40(10):1649-53.
26- Sheykhrezai MS, Aligholi M, Ghorbanzadeh R, Bahador A. A comparative study of antimicrobial activity of Proroot MTA, Root MTA, and Portland cement on Actinobacillus Actinomycetemcomitans. Iran Endod J. 2008 Fall;3(4):129-33.
27- Ribeiro DA, Matsumoto MA, Duarte MA, Marques ME, Salvadori DM. Ex vivo biocompatibility tests of regular and white forms of mineral trioxide aggregate. Int Endod J. 2006 Jan;39(1):26-30.
28- Cabiscol E, Tamarit J, Ros J. Oxidative stress in bacteria and protein damage by reactive oxygen species. Int Microbiol. 2000 Mar;3(1):3-8.
29- Ribeiro CS, Scelza MF, Hirata Júnior R, Buarque de Oliveira LM. The antimicrobial activity of gray-colored mineral trioxide aggregate (GMTA) and white-colored MTA (WMTA) under aerobic and anaerobic conditions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Jun;109(6):e109-12.
30- Camilleri J, Montesin FE, Brady K, Sweeney R, Curtis RV, Ford TR. The constitution of mineral trioxide aggregate. Dent Mater. 2005 Apr;21(4):297-303.
31- Dammaschke T, Gerth HU, Züchner H, Schäfer E. Chemical and physical surface and bulk material characterization of white ProRoot MTA and two Portland cements. Dent Mater. 2005 Aug;21(8):731-8.
IssueVol 15, No 5 (2018) QRcode
SectionOriginal Article
Bacterial Sensitivity Test Mineral Trioxide Aggregate OrthoMTA ProRoot MTA RetroMTA

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How to Cite
Khedmat S, Aminipor M, Pourhajibagher M, Kharazifar MJ, Bahador A. Comparison of Antibacterial Activities of ProRoot MTA, OrthoMTA, and RetroMTA Against Three Anaerobic Endodontic Bacteria. Front Dent. 2018;15(5):294-299.