Original Article

Comparison of the Antibacterial Effect of AH26, Adseal and Beta RCS Root Canal Sealers Against Enterococcus Faecalis, an in Vitro Study

An in Vitro Study

Abstract

Objectives: Antibacterial activity against endodontic pathogens is a desirable feature for root canal sealers. The objective of this study was to compare the antibacterial effect of three resin-based endodontic sealers (AH26, Adseal, and Beta RCS) against Enterococcus faecalis in vitro.

Materials and Methods: The antibacterial properties of the sealers were assessed against E. faecalis using agar diffusion test (ADT) for fresh state (N=10) and direct contact test (DCT) for freshly-mixed and set states of the materials (N=10). In ADT, the diameter of the zones of inhibition was measured after 24h of contact. In DCT, the colony-forming units of the bacteria were counted after 30 minutes and 180 minutes of exposure. The results were analyzed with two-way ANOVA and independent sample t-test. P<0.05 was considered significant.

Results: Regarding DCT results, all test materials indicated an antibacterial effect, both in freshly-mixed and set states. The highest antibacterial effect was related to Adseal, whereas the lowest was observed in Beta RCS. There was a significant difference between all study groups (different sealers, setting states, and contact times; P<0.001), except for freshly-mixed AH26 and Adseal at 180 minutes (P>0.05). According to ADT, AH26 and Adseal represented the widest and the smallest inhibition zones, respectively (P<0.001).

Conclusion: Within the limitations of this in vitro study, AH26, Adseal, and Beta RCS showed antibacterial effects against E. faecalis in both freshly-mixed and set states. The antibacterial effect increased over time in all of the studied sealers.

1. Karamifar K, Tondari A, Saghiri MA. Endodontic Periapical Lesion: An Overview on the Etiology, Diagnosis and Current Treatment Modalities. Eur Endod J. 2020;5(2):54-67.
2. Rôças IN, Siqueira Jr JF. In vivo antimicrobial effects of endodontic treatment procedures as assessed by molecular microbiologic techniques. J Endod. 2011;37(3):304-10.
3. Sakamoto M, Siqueira Jr J, Rôças I, Benno Y. Molecular analysis of the root canal microbiota associated with endodontic treatment failures. Oral Microbiol Immunol. 2008;23(4):275-81.
4. Zargar N, Rayat Hosein Abadi M, Sabeti M, Yadegari Z, Akbarzadeh Baghban A, Dianat O. Antimicrobial efficacy of clindamycin and triple antibiotic paste as root canal medicaments on tubular infection: An in vitro study. Aust Endod J. 2019;45(1):86-91.
5. Sunde PT, Olsen I, Göbel UB, Theegarten D, Winter S, Debelian GJ, et al. Fluorescence in situ hybridization (FISH) for direct visualization of bacteria in periapical lesions of asymptomatic root-filled teeth. Microbiology. 2003;149(5):1095-102.
6. Silva EJ, Cardoso ML, Rodrigues JP, De-Deus G, Fidalgo TKdS. Solubility of bioceramic-and epoxy resin-based root canal sealers: A systematic review and meta-analysis. Aust Endod J. 2021;47(3):690-702.
7. Siqueira Jr JF, Gonçalves RB. Antibacterial activities of root canal sealers against selected anaerobic bacteria. J Endod. 1996;22(2):79-80.
8. AlShwaimi E, Bogari D, Ajaj R, Al-Shahrani S, Almas K, Majeed A. In vitro antimicrobial effectiveness of root canal sealers against Enterococcus faecalis: a systematic review. J Endod. 2016;42(11):1588-97.
9. Ehsani M, Adibi A, Moosavi E, Dehghani A, Khafri S, Adibi E. Antimicrobial activity of three different endodontic sealers on the enterococcus faecalis and lactobacillus (in vitro). Caspian J Dent Res. 2013;2(2):8-14.
10. Kangarlou A, Neshandar R, Matini N, Dianat O. Antibacterial efficacy of AH Plus and AH26 sealers mixed with amoxicillin, triple antibiotic paste and nanosilver. Journal of dental research, dental clinics, dental prospects. 2016;10(4):220-5.
11. Standardization of ISO for dentistry. Dental Root Canal Sealing Materials. Geneva, Switzerland; 2012.
12. Nair P. On the causes of persistent apical periodontitis: a review. Int Endod J. 2006;39(4):
249-81.
13. Liu D, Peng X, Wang S, Han Q, Li B, Zhou X, et al. A novel antibacterial resin-based root canal sealer modified by Dimethylaminododecyl Methacrylate. Sci Rep. 2019;9(1):1-9.
14. Huang Y, Li X, Mandal P, Wu Y, Liu L, Gui H, et al. The in vitro antimicrobial activities of four endodontic sealers. BMC oral health. 2019;19(1):1-7.
15. Standardization of ISO for dentistry 7405. Evaluation of Biocompatibility of Medical Devices Used in Dentistry. Geneva, Switzerland; 2018.
16. Weiss E, Shalhav M, Fuss Z. Assessment of antibacterial activity of endodontic sealers by a direct contact test. Dent Traumatol. 1996;12(4):179-84.
17. Milani AS, Moeinian A, Barhaghi MHS, Abdollahi AA. Evaluation of the film thickness and antibacterial property of mineral trioxide aggregate mixed with propylene glycol as a root canal sealer. Dent Res J. 2020;17(2):142-6.
18. Bubonja-Šonje M, Knežević S, Abram M. Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds. Arh Hig Rada Toksikol. 2020;71(4):300-11.
19. Shakouie S, Esk M, Shahi S, FroughReihani M, Soroush M, Gosili A. Antimicrobial efficacy of AH-Plus, adseal and endofill against Enterococcus faecalis-An in vitro study. Afr J Microbiol Res. 2012;6(5):991-4.
20. Simsek M, Kanik O. Antimicrobial activity of root canal sealers against some standard strains and clinical isolates. Medicine. 2021;10(2):586-91.
21. Song Y-S, Choi Y, Lim M-J, Yu M-K, Hong C-U, Lee K-W, et al. In vitro evaluation of a newly produced resin-based endodontic sealer. Restor Dent Endod. 2016;41(3):189-95.
22. Dash AK, Dash A, Thakur JS, Farista S, Asrani H, Rathi S. Comparative evaluation of flow rate of four different endodontic sealers: an in vitro study. Endodontology. 2020;32(2):96-9.
23. Abu Zeid S, Mokeem Saleh A. Solubility, pH Changes and releasing elements of different bioceramic and mineral trioxide aggregate root canal sealers comparative study. J Trauma Treat. 2015;4(249):2167-1222.
24. Lorencová E, Vltavská P, Budinský P, Koutný M. Antibacterial effect of phosphates and polyphosphates with different chain length. J Environ Sci Health. 2012;47(14):2241-5.
24. Lorencová E, Vltavská P, Budinský P, Koutný M. Antibacterial effect of phosphates and polyphosphates with different chain length. Journal of Environmental Science and Health, Part A. 2012 Dec 1;47(14):2241-5.
IssueVol 21 (Continuously Published Article-Based) QRcode
SectionOriginal Article
DOI https://doi.org/10.18502/fid.v21i5.14853
Keywords
Anti-Bacterial Agents Bacteria Epoxy Resin-Based Root Canal Sealer Root Canal Filling Materials
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
Ghabraei S, Assadian H, Razmi H, Sheikhrezaei MS, Khedma S, Chitsaz N, Mohammadi Z, Noori F. Comparison of the Antibacterial Effect of AH26, Adseal and Beta RCS Root Canal Sealers Against Enterococcus Faecalis, an in Vitro Study. Front Dent. 2024;21.